The mechanism by which Enteromorpha Linza polysaccharide promotes Bacillus subtilis growth and nitrate removal

doi:10.1016/j.ijbiomac.2022.04.082

IOCAS-IR > 实验海洋生物学重点实验室

	The mechanism by which Enteromorpha Linza polysaccharide promotes Bacillus subtilis growth and nitrate removal
	Zhang, Hui1,2,4,5 ; Chen, Xiaolin 1,3,5; Song, Lin2 ; Liu, Song 1,3,5; Li, Pengcheng1,3,5
	2022-06-01
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	209 页码:840-849
通讯作者	Chen, Xiaolin(chenxl@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
摘要	In this study, we discussed the relationship between Entermorpha linza polysaccharide (EP) and Bacillus subtilis, which can transform nitrate. A sole carbon source experiment showed that Bacillus subtilis could utilize EP, and the bacterial density was maximally increased by 54.43% in the EP groups. The results of reducing sugar determination proved the secretion of polysaccharide-degrading enzymes. Scanning electron microscopy (SEM) showed that the EP groups had fewer spores and shrunken bacteria, indicating that EP could improve the growth environment and maintain bacterial integrity. Additionally, the ratios of periplasmic nitrate reductase (NAP), nitrite reductase (NIR), and dissimilatory nitrate reductase (D-NRase) in the EP groups were maximally increased by 107.22%, 84.70% and 36.10%, respectively. Transcriptome analysis further confirmed the above mentioned results. For example, the high expression of quorum sensing genes indicated that EP groups had higher bacterial density. Moreover, the high expression of antioxidant genes in the EP groups may be related to morphological integrity. Our study provides a basis for further discussion of the mechanism.
关键词	Enteromorpha Linza polysaccharide Bacillus subtilis Growth promotion Nitrate removal Mechanism
DOI	10.1016/j.ijbiomac.2022.04.082
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0901104]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:000796998300001
出版者	ELSEVIER
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179345
专题	实验海洋生物学重点实验室
通讯作者	Chen, Xiaolin; Li, Pengcheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Hui,Chen, Xiaolin,Song, Lin,et al. The mechanism by which Enteromorpha Linza polysaccharide promotes Bacillus subtilis growth and nitrate removal[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2022,209:840-849.
APA	Zhang, Hui,Chen, Xiaolin,Song, Lin,Liu, Song,&Li, Pengcheng.(2022).The mechanism by which Enteromorpha Linza polysaccharide promotes Bacillus subtilis growth and nitrate removal.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,209,840-849.
MLA	Zhang, Hui,et al."The mechanism by which Enteromorpha Linza polysaccharide promotes Bacillus subtilis growth and nitrate removal".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 209(2022):840-849.

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