Identification and characterization of olfactory receptor genes and olfactory perception in rapa whelk Rapana venosa (Valenciennes, 1846) during larval settlement and metamorphosis

doi:10.1016/j.gene.2022.146403

	Identification and characterization of olfactory receptor genes and olfactory perception in rapa whelk Rapana venosa (Valenciennes, 1846) during larval settlement and metamorphosis
	Yu, Zheng-Lin 1,4; Feng, Jie 2,3,4,5,7; Song, Hao 2,3,4,5,7; Yang, Mei-Jie 2,3,4,5,7; Hu, Zhi 2,3,4,5,6,7; Zhou, Cong2,3,4,5,6,7 ; Shi, Pu2,3,4,5,6,7 ; Yuan, Xiu-Tang 1,4; Li, Hai-Zhou 8; Zhang, Tao2,3,4,5,7 ; Liu, Lei 9
	2022-05-30
发表期刊	GENE
ISSN	0378-1119
卷号	825 页码:8
通讯作者	Zhang, Tao(tzhang@qdio.ac.cn)
摘要	The rapa whelk Rapana venosa, an economically important marine fishery resource in China but a major invader all over the world, changes from a phytophagous to a carnivorous form following settlement and metamorphosis. However, the low settlement and metamorphosis rates (<1%) of larvae limit the abundance of R. venosa. This critical step (settlement and metamorphosis) remains poorly characterized but may be related to how larvae perceive the presence of shellfish, their new source of food. Here, we report that larvae may use olfactory perception to sense shellfish. Olfactory receptor (OR) genes are involved in odor sensing in animals. We identified a total of 463 OR genes, which could be grouped into nine clades based on phylogenetic analysis. When assessing the attraction of larvae at different developmental stages to oyster odor, R. venosa showed active settlement and metamorphosis behavior only at the J4 stage (competent larva, 1000-1500 mu m shell length) and in the presence of shellfish odor at the same time. Expression of OR gene family members differed between stage 2 (four-spiral whorl stage) and stage 1 (single-to three-spiral whorl stage), indicating significant changes in the olfactory system during larval development. These findings broaden our understanding of olfactory perception, settlement, and metamorphosis in gastropods and can be used to improve R. venosa harvesting, as well as the sustainable development and utilization of this resource.
关键词	Olfactory perception Rapana venosa OR gene family Settlement and metamorphosis
DOI	10.1016/j.gene.2022.146403
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[32002374] ; National Natural Science Foundation of China[32002409] ; National Natural Science Foundation of China[31972814] ; Natural Science Foundation of Shandong Province[ZR2019BD003] ; China Postdoctoral Science Foundation[2019 M652498] ; Major Scientific and Technological Innovation Project of the Shandong Provincial Key Research and Development Program[2019JZZY020708] ; China Agriculture Research System of MOF and MARA ; Industry Leading Talents Project of Taishan Scholars[LJNY201704] ; 'Double Hundred' Blue Industry Leader Team of Yantai ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000792441100001
出版者	ELSEVIER
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179189
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Tao
作者单位	1.Chinese Acad Sci, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, CAS Engn Lab Marine Ranching, Inst Oceanol, Qingdao 266071, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 8.Shandong Fu Han Ocean Sci Tech Co Ltd, Haiyang 265114, Peoples R China 9.Genepioneer Biotechnol Co Ltd, Nanjing 210000, Jiangsu, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心; 中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yu, Zheng-Lin,Feng, Jie,Song, Hao,et al. Identification and characterization of olfactory receptor genes and olfactory perception in rapa whelk Rapana venosa (Valenciennes, 1846) during larval settlement and metamorphosis[J]. GENE,2022,825:8.
APA	Yu, Zheng-Lin.,Feng, Jie.,Song, Hao.,Yang, Mei-Jie.,Hu, Zhi.,...&Liu, Lei.(2022).Identification and characterization of olfactory receptor genes and olfactory perception in rapa whelk Rapana venosa (Valenciennes, 1846) during larval settlement and metamorphosis.GENE,825,8.
MLA	Yu, Zheng-Lin,et al."Identification and characterization of olfactory receptor genes and olfactory perception in rapa whelk Rapana venosa (Valenciennes, 1846) during larval settlement and metamorphosis".GENE 825(2022):8.

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