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A newly identified NLR-like gene participates in bacteria and virus infection possibly through regulating hemocytes apoptosis in shrimp
Li, Xuechun1,2,3,6; Li, Shihao1,2,4,6; Sun, Mingzhe1,6; Yu, Yang1,2,4,6; Zhang, Xiaojun1,2,4,6; Xiang, Jianhai1,2,6; Li, Fuhua1,2,4,5,6
2022-07-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume132Pages:10
Corresponding AuthorLi, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
AbstractThe nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) play important roles in innate immunity. Previously, we identified an NLR-like gene, LvNLRPL1, and found that it participated in Vibrio infection and regulated hemocytes apoptosis in the Pacific whiteleg shrimp Litopenaeus vannamei. However, it is still unclear whether other NLR-like genes exist in shrimp and how they function during virus infection. In the present study, a novel NLR-like gene (LvNLRPL2) was identified and functionally characterized in L. vannamei. LvNLRPL2 was highly expressed in hemocytes and responsive to both Vibrio parahaemolyticus and white spot syndrome virus (WSSV) infection. Knockdown of LvNLRPL2 could markedly increase the proliferation of Vibrio and the mortality of shrimp infected with V. parahaemolyticus, whereas inhibit in vivo WSSV propagation in shrimp, indicating its distinct roles during Vibrio and WSSV infection. After LvNLRPL2 knockdown, the apoptotic rate of hemocytes increased, and the expression levels of LvCaspase 2, 3 and 5 were significantly up-regulated. In addition, LvNLRPL2 could form a hetero-dimer with LvNLRPL1 through their NACHT domains. These results suggest that LvNLRPL2 might resist bacterial infection while promote WSSV propagation by forming heterodimer with LvNLRPL1 and then inhibiting apoptosis of hemocytes. These data will be helpful for understanding the functions of NLR-like genes and their regulation mechanisms in crustaceans.
KeywordNLRs Immune response Apoptosis Vibrio parahaemolyticus White spot syndrome virus
DOI10.1016/j.dci.2022.104395
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31972829] ; National Natural Science Foundation of China[31830100] ; National Natural Science Foundation of China[41776158] ; China Agriculture Research System of MOF and MARA
WOS Research AreaFisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS SubjectFisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS IDWOS:000791225000001
PublisherELSEVIER SCI LTD
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/178997
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Shihao; Li, Fuhua
Affiliation1.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
5.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
6.Chinese Acad Sci, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Xuechun,Li, Shihao,Sun, Mingzhe,et al. A newly identified NLR-like gene participates in bacteria and virus infection possibly through regulating hemocytes apoptosis in shrimp[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2022,132:10.
APA Li, Xuechun.,Li, Shihao.,Sun, Mingzhe.,Yu, Yang.,Zhang, Xiaojun.,...&Li, Fuhua.(2022).A newly identified NLR-like gene participates in bacteria and virus infection possibly through regulating hemocytes apoptosis in shrimp.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,132,10.
MLA Li, Xuechun,et al."A newly identified NLR-like gene participates in bacteria and virus infection possibly through regulating hemocytes apoptosis in shrimp".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 132(2022):10.
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