iTRAQ-based proteome profiling revealed the carbon metabolism regulation for mariculture wastewater treatment with Bacterial-Algal Coupling Technology

doi:10.1016/j.cej.2022.135579

IOCAS-IR

	iTRAQ-based proteome profiling revealed the carbon metabolism regulation for mariculture wastewater treatment with Bacterial-Algal Coupling Technology
	Gao, Pengtao 1,2; Gao, Yedong 1,3; Guo, Liang 1,4; Gao, Mengchun 1; Zhao, Yangguo 1; Jin, Chunji 1; She, Zonglian 1; Wang, Guangce 5
	2022-07-01
发表期刊	CHEMICAL ENGINEERING JOURNAL
ISSN	1385-8947
卷号	439 页码:13
通讯作者	Guo, Liang(geletu@ouc.edu.cn)
摘要	Acidogenic fermentation coupled with microalgae cultivation is efficient for mariculture wastewater (MW) treatment and energy recovery, but there is limited information regarding carbon metabolic networks as a response to varied broth composition at the proteome level. In this study, ethanol-type, propionate-type, and butyrate-type fermentation effluent of MW were applied to microalgae cultivation. The carbon source compo-sition of different types of acidogenic fermentation was optimized to enhance microalgal biomass production and nutrients removal. Compared with ethanol-type fermentation effluent, higher efficiency in biomass accumulation and ammonia assimilation of Chlorella pyrenoidosa was observed in propionate-type and butyrate-type. Lipid accumulation in microalgae was enhanced with ethanol-type fermentation effluent, and the maximum lipid content reached 513.3 and 546.7 mg/L at an inorganic carbon/organic carbon (IC/OC) ratio of 3:1 and 2:1, respectively. The increasing ratio of IC/OC improved the expression of key enzymes related to the photosynthetic system, which had a positive effect on carbohydrate production and microalgal growth. The upregulation of magnesium chelatase with bicarbonate supply was revealed by iTRAQ analysis, thereby promoting chlorophyll production in microalgal cells. In response to an increasing proportion of IC, more energy and precursor sub-stances for lipid production were generated with the enhancing expression of glycolysis and pyruvate metabolism.
关键词	Acidogenic fermentation Microalgae cultivation Carbon metabolism regulation iTRAQ-based proteome Mariculture wastewater treatment
DOI	10.1016/j.cej.2022.135579
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[52070173] ; Qingdao Science and Technology Benefit People Demonstration and Guidance Project[21-1-4-sf-12-nsh] ; Fundamental Research Funds for the Central Universities[201964005] ; Urban and Rural Housing Construction Science and Technology Project of Shan-dong[2021-K8-7]
WOS研究方向	Engineering
WOS类目	Engineering, Environmental ; Engineering, Chemical
WOS记录号	WOS:000783569800004
出版者	ELSEVIER SCIENCE SA
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178991
专题	中国科学院海洋研究所
通讯作者	Guo, Liang
作者单位	1.Ocean Univ China, Coll Environm Sci & Engn, Qingdao 266100, Peoples R China 2.Katholieke Univ Leuven, Dept Chem Engn, Celestijnenlaan 200F, B-3001 Leuven, Belgium 3.Harbin Inst Technol, Sch Environm, State Key Lab Urban Water Resource & Environm SKL, Harbin 150090, Peoples R China 4.Ocean Univ China, Minist Educ, Key Lab Marine Environm & Ecol, Qingdao 266100, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Gao, Pengtao,Gao, Yedong,Guo, Liang,et al. iTRAQ-based proteome profiling revealed the carbon metabolism regulation for mariculture wastewater treatment with Bacterial-Algal Coupling Technology[J]. CHEMICAL ENGINEERING JOURNAL,2022,439:13.
APA	Gao, Pengtao.,Gao, Yedong.,Guo, Liang.,Gao, Mengchun.,Zhao, Yangguo.,...&Wang, Guangce.(2022).iTRAQ-based proteome profiling revealed the carbon metabolism regulation for mariculture wastewater treatment with Bacterial-Algal Coupling Technology.CHEMICAL ENGINEERING JOURNAL,439,13.
MLA	Gao, Pengtao,et al."iTRAQ-based proteome profiling revealed the carbon metabolism regulation for mariculture wastewater treatment with Bacterial-Algal Coupling Technology".CHEMICAL ENGINEERING JOURNAL 439(2022):13.

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