IOCAS-IR  > 实验海洋生物学重点实验室
Characterization of the First Cultured Representative of "Candidatus Thermofonsia" Clade 2 within Chloroflexi Reveals Its Phototrophic Lifestyle
Zheng, Rikuan1,2,3,4,5; Cai, Ruining1,2,3,4,5,6; Wang, Chong1,2,3,4,5; Liu, Rui1,2,3,4,5; Sun, Chaomin1,2,3,4,5,6
2022-04-26
Source PublicationMBIO
ISSN2150-7511
Volume13Issue:2Pages:17
Corresponding AuthorSun, Chaomin(sunchaomin@qdio.ac.cn)
Abstract"Candidatus Thermofonsia" represents a novel class within the phylum Chloroflexi. Metagenomic analysis reveals "Ca. Thermofonsia" harbors phototrophs outside the classically phototrophic Chloroflexia class. Unfortunately, the paucity of pure cultures limits further insights into their potential phototrophy. Here, we report the successful isolation of a "Ca. Thermofonsia" representative (Phototrophicus methaneseepsis ZRK33) from a deep-sea cold seep. Using combined physiological, genomic, and transcriptomic methods, we further show the long-wavelength light (e.g., red and infrared light) could promote the growth of strain ZRK33 and upregulate the expression of genes associated with phototrophy. In particular, strain ZRK33 has a typical phototrophic lifestyle under both laboratory and deep-sea conditions. Strain ZRK33 also possesses the ability to fix inorganic carbon through the 3-hydroxypropionate bicycle in both laboratory and deep-sea in situ environments, and the combined autotrophic, phototrophic, and heterotrophic capabilities endow strain ZRK33 with a photomixotrophic lifestyle. Notably, the predicted genes associated with phototrophy broadly exist in the metagenomes of 27 deep-sea Chloroflexi members, strongly suggesting diverse phototrophic Chloroflexi members are distributed in various unexplored deep biospheres. IMPORTANCE The deep ocean microbiota represents the unexplored majority of global ocean waters. The phylum Chloroflexi is abundant and broadly distributed in various deep-sea ecosystems. It was reported that some members of "Candidatus Thermofonsia" clade 2 might possess phototrophs; however, the absence of cultured representatives is a significant bottleneck toward understanding their phototrophic characteristics. In the present study, we successfully isolated a representative of the novel class "Ca. Thermofonsia" from a deep-sea cold seep by using an enrichment medium constantly supplemented with rifampicin, allowing researchers to isolate more Chloroflexi members in the future. Importantly, outside the classically phototrophic Chloroflexia class, we discover a novel phototrophic clade within the phylum Chloroflexi and demonstrate the existence of phototrophic lifestyles in the deep sea. Thus, this study expands the range of phototrophic Chloroflexi and provides a good model to study the mechanism of phototrophy performed in the deep biosphere. The deep ocean microbiota represents the unexplored majority of global ocean waters. The phylum Chloroflexi is abundant and broadly distributed in various deep-sea ecosystems.
KeywordChloroflexi "Candidatus Thermofonsia" deep sea phototrophic lifestyle photosynthetic reaction center
DOI10.1128/mbio.00287-22
Indexed BySCI
Language英语
Funding ProjectStrategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; China Ocean Mineral Resources R&D Association Grant[DY135-B2-14] ; Key Deployment Projects of Center of Ocean Mega-Science of the Chinese Academy of Sciences[COMS2020Q04] ; Major Research Plan of the National Natural Science Foundation[92051107] ; National Key R and D Program of China[2018YFC0310800] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051]
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000788061200068
PublisherAMER SOC MICROBIOLOGY
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/178946
Collection实验海洋生物学重点实验室
Corresponding AuthorSun, Chaomin
Affiliation1.Chinese Acad Sci, CAS, Inst Oceanol, Qingdao, Peoples R China
2.Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China
3.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
6.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zheng, Rikuan,Cai, Ruining,Wang, Chong,et al. Characterization of the First Cultured Representative of "Candidatus Thermofonsia" Clade 2 within Chloroflexi Reveals Its Phototrophic Lifestyle[J]. MBIO,2022,13(2):17.
APA Zheng, Rikuan,Cai, Ruining,Wang, Chong,Liu, Rui,&Sun, Chaomin.(2022).Characterization of the First Cultured Representative of "Candidatus Thermofonsia" Clade 2 within Chloroflexi Reveals Its Phototrophic Lifestyle.MBIO,13(2),17.
MLA Zheng, Rikuan,et al."Characterization of the First Cultured Representative of "Candidatus Thermofonsia" Clade 2 within Chloroflexi Reveals Its Phototrophic Lifestyle".MBIO 13.2(2022):17.
Files in This Item:
File Name/Size DocType Version Access License
mbio.00287-22.pdf(4433KB)期刊论文出版稿限制开放CC BY-NC-SAView
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zheng, Rikuan]'s Articles
[Cai, Ruining]'s Articles
[Wang, Chong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zheng, Rikuan]'s Articles
[Cai, Ruining]'s Articles
[Wang, Chong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zheng, Rikuan]'s Articles
[Cai, Ruining]'s Articles
[Wang, Chong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: mbio.00287-22.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.