Validation of mating type-related markers in Ulva prolifera (Ulvophyceae, Chlorophyta) and their detection during various reproductive modes

doi:10.1016/j.algal.2021.102611

IOCAS-IR > 实验海洋生物学重点实验室

	Validation of mating type-related markers in Ulva prolifera (Ulvophyceae, Chlorophyta) and their detection during various reproductive modes
	Liu, Qianchun 1,2,3,4; Wu, Chunhui1,2,3 ; Xie, Weifeng 1,2,3,4; Zhao, Jin 1,2,3; Jiang, Peng 1,2,3
	2022-03-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN	2211-9264
卷号	62 页码:9
通讯作者	Jiang, Peng(jiangpeng@qdio.ac.cn)
摘要	Ulva prolifera dominates the world's largest green tide in the Yellow Sea. In addition to the typical sexual life cycle, which is diplohaplontic and isomorphic, U. prolifera can reproduce in a variety of asexual ways, and its reproductive strategy contributes significantly to the blooms. However, survey statistics on the life cycle dynamics have been seriously restricted owing to difficulties in morphologically determining the ploidy or sex of thalli, as well as the lack of reliable molecular markers. Here, two mating type (MT)-specific markers were modified based on the published MT-locus gene of U. prolifera, and validated in a sexual life cycle. The two markers paired in diploid sporophytes and segregated in haploid gametophytes, with mating occurring only between those with different markers, which confirmed that these markers were allelic and MT-related in U. prolifera. Furthermore, the ploidy variations in three types of reproduction, parthenogenesis, apomeiosis, and so-called somatic cell germination were determined in U. prolifera. No ploidy change was detected using flow cytometry between parents and progeny, and this was confirmed using these markers. In addition, the somatic cell germination process was characterized in detail. It is essentially a process of vegetative reproduction-in situ somatic embryogenesis, and it may occur in both sporophytes and gametophytes of U. prolifera. In U. prolifera, these MT-related markers can determine the ploidy or sex of thalli in one step, and the rules determining marker transmission along during the reported reproductive pathways were elucidated. This marker pair is an important molecular tool that can be used in future studies on the reproductive strategy of U. prolifera during green tides.
关键词	Apomeiosis Mating type-related marker Parthenogenesis In situ somatic embryogenesis Ulva prolifera
DOI	10.1016/j.algal.2021.102611
收录类别	SCI
语种	英语
资助项目	Science & Technology Basic Resources Investigation Program of China[2018FY100205] ; Young Scientists Fund of LMBB ; Qingdao National Laboratory for Marine Science and Technology[YQ2018NO09] ; National Natural Science Foundation of China[41776153] ; Key Deployment Project of Centre for Ocean Mega-Research of Science ; Chinese Academy of Science[COMS2019Q05] ; Key R&D Program of Shandong Province[2019GSF107012] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050302]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000787890400014
出版者	ELSEVIER
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178922
专题	实验海洋生物学重点实验室
通讯作者	Jiang, Peng
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Liu, Qianchun,Wu, Chunhui,Xie, Weifeng,et al. Validation of mating type-related markers in Ulva prolifera (Ulvophyceae, Chlorophyta) and their detection during various reproductive modes[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,62:9.
APA	Liu, Qianchun,Wu, Chunhui,Xie, Weifeng,Zhao, Jin,&Jiang, Peng.(2022).Validation of mating type-related markers in Ulva prolifera (Ulvophyceae, Chlorophyta) and their detection during various reproductive modes.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,62,9.
MLA	Liu, Qianchun,et al."Validation of mating type-related markers in Ulva prolifera (Ulvophyceae, Chlorophyta) and their detection during various reproductive modes".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 62(2022):9.

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