The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida

doi:10.3390/pathogens11040464

IOCAS-IR > 实验海洋生物学重点实验室

	The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida
	Liu, Xin1,2,3,4 ; Wang, Xinhui 1,2; Sun, Boguang1,2,3 ; Sun, Li 1,2,3,4
	2022-04-01
发表期刊	PATHOGENS
卷号	11 期号:4 页码:16
通讯作者	Sun, Boguang(sunboguang@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn)
摘要	Edwardsiella piscicida is a pathogenic bacterium, which can infect a number of fish species and cause a disease termed edwardsiellosis, threatening global fish farming with high prevalence and mortality. Thiamine (Vitamin B1), functioning in the form of thiamine pyrophosphate (TPP), is essential for almost all organisms. Bacteria acquire TPP by biosynthesis or by transportation of exogenous thiamine. TPP availability has been associated with bacterial pathogenicity, but the underlying mechanisms remain to be discovered. The role of thiamine in the pathogenicity of E. piscicida is unknown. In this study, we characterized a thiamine transporter (TT) operon in E. piscicida. The deletion of the TT operon resulted in an intracellular TPP lacking situation, which led to attenuated overall pathogenicity, impaired abilities associated with motility and host cell adhesion, as well as decreased expression of certain flagellar and adhesion genes. Moreover, TPP starvation led to intracellular c-di-GMP reduction, and introducing into the TPP-suppressed mutant strain an exogenous diguanylate cyclase for c-di-GMP synthesis restored the virulence loss. Taken together, this work reveals the involvement of thiamine uptake in the virulence regulation of E. piscicida, with c-di-GMP implicated in the process. These finding could be employed to explore potential drug targets against E. piscicida.
关键词	Edwardsiella piscicida thiamine transporter c-di-GMP virulence
DOI	10.3390/pathogens11040464
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0900500] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000786725800001
出版者	MDPI
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178883
专题	实验海洋生物学重点实验室
通讯作者	Sun, Boguang; Sun, Li
作者单位	1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266003, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Liu, Xin,Wang, Xinhui,Sun, Boguang,et al. The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida[J]. PATHOGENS,2022,11(4):16.
APA	Liu, Xin,Wang, Xinhui,Sun, Boguang,&Sun, Li.(2022).The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida.PATHOGENS,11(4),16.
MLA	Liu, Xin,et al."The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida".PATHOGENS 11.4(2022):16.

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