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Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae
Li, Jiang1; Gu, Xiaoqian2; Zhang, Qian1; Fu, Liping1; Tan, Jiaojiao2; Zhao, Luying1
2022-03-31
发表期刊FRONTIERS IN MICROBIOLOGY
卷号13页码:11
通讯作者Li, Jiang(lijiang@fio.org.cn)
摘要A carrageenase gene, car1383, was obtained from the metagenome of Antarctic macroalgae-associated bacteria. The amino acid sequence of its product showed up to 33% similarity with other carrageenases and contained a GH16-family motif. The recombinant Car1383 was heterologously expressed in Eschericia coli and exhibited maximal activity at 50 degrees C and pH 6.0, with a K-m of 6.51 mg/ml and a V-max of 55.77 U/mg. Its activity was enhanced by some cations (Na+, K+, and Fe2+), but inhibited or inactivated by others (Sr2+, Ca2+, Ni2+, Ba2+, Mn2+, Cu2+, Fe3+, and Mg2+). Car1383 degraded carrageenan into neocarrabiose and neocarratetraose. Site-directed mutagenesis indicated that putative active sites, E-190 and E-195, conserved sites, W-183 and G(255), play important roles in Car1383 activity. This study provides a new candidate for the industrial preparation of bioactive algal oligosaccharides.
关键词carrageenase Antarctic macroalgae associated bacteria metagenome site-directed mutagenesis
DOI10.3389/fmicb.2022.851182
收录类别SCI
语种英语
资助项目Impact and Response of Antarctic Seas to Climate Change[RFSOCC2020-2022]
WOS研究方向Microbiology
WOS类目Microbiology
WOS记录号WOS:000789133600001
出版者FRONTIERS MEDIA SA
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/178877
专题实验海洋生物学重点实验室
通讯作者Li, Jiang
作者单位1.Minist Nat Resources, Inst Oceanog 1, Key Lab Ecol Environm Sci & Technol, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
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Li, Jiang,Gu, Xiaoqian,Zhang, Qian,et al. Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae[J]. FRONTIERS IN MICROBIOLOGY,2022,13:11.
APA Li, Jiang,Gu, Xiaoqian,Zhang, Qian,Fu, Liping,Tan, Jiaojiao,&Zhao, Luying.(2022).Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae.FRONTIERS IN MICROBIOLOGY,13,11.
MLA Li, Jiang,et al."Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae".FRONTIERS IN MICROBIOLOGY 13(2022):11.
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