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Ubiquitin-proteasome pathway plays an essential regulatory role during spermatangium formation in Neopyropia yezoensis
Niu, Jianfeng1,2,3; Yang, Jiali1,4; Feng, Jianhua1; Feng, Zezhong1,5; Wang, Xia1,4; Yu, Bin1,4; Wang, Guangce1,2,3
2022-03-01
发表期刊ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
卷号62页码:12
通讯作者Wang, Guangce(gcwang@qdio.ac.cn)
摘要Gerproductive cell formation is one of the most important physiological processes of organisms. The Rhodophyta, Neopyropia yezoensis, is a typical Archaeplastida and the spermatangia is usually formed along the edge of thalli and visible to the naked eye. It is therefore meaningful to invest the mechanisms of cell development. Here, the cell clumps at four typical differentiation stages of spermatangium in N. yezoensis were collected and transcriptome analysis was performed. A total of 12,188 differentially expressed genes (DEGs) were identified and the number of DEGs increased compared to the control as spermatangium formation progressed. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that carbon fixation, glycolysis/gluconeogenesis, photosynthesis-antenna proteins and some primary metabolic processes were down-regulated. In contrast, sphingolipid metabolism, inositol phosphate metabolism, ubiquitin mediated proteolysis and DNA replication were up-regulated. The development of spermatangia is believed to be induced by the sex signaling molecules due to the up-regulation of sphingolipid metabolism, the biosynthesis of phytosterol and the expression of a steroid bound receptor of heme-binding b5-domain containing protein at the initial stage of spermatangia formation. During the course of development, a total of 163 DEGs related to the ubiquitin-proteasome (Ub-proteasome) system were identified and half of these were up-regulated. Additionally, a series of ubiquitin-protein ligases (E3s) related to development, cell cycle, and chromosome replication were analyzed by qRT-PCR. The continuously upregulated of histone H2A/H2B implied that the shift of global gene expression profile modulated by the Ub-proteasome system was necessary during the differentiation. The up-regulation of the anaphasepromoting complex (APC) and its related protein factors meant that ubiquitin-mediated proteolysis enhanced the cell-cycle and chromosome replication. All the results suggest that the development of male germ cells is a highly modulated process and the Ub-proteasome system might play an essential regulatory role.
关键词Neopyropia yezoensis Ubiquitin 26S proteasome Sexual differentiation Spermatangium Molecular mechanism
DOI10.1016/j.algal.2021.102623
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[41776150] ; National Natural Science Foundation of China[41476140] ; China Agriculture Research System of MOF ; Key Deployment Project of the Centre for OceanMega-Research of Science ; Chinese Academy of Sciences ; Major Scientific and Technological Innova-tion Project of Shandong Provincial Key Research and Development Program ; [COMS2019Q02] ; [2019JZZY010815]
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000787890400009
出版者ELSEVIER
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/178874
专题实验海洋生物学重点实验室
通讯作者Wang, Guangce
作者单位1.Chinese Acad Sci IOCAS, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, 168 Wenhai Middle Rd, Qingdao 266237, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao 266109, Peoples R China
第一作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
通讯作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
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Niu, Jianfeng,Yang, Jiali,Feng, Jianhua,et al. Ubiquitin-proteasome pathway plays an essential regulatory role during spermatangium formation in Neopyropia yezoensis[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,62:12.
APA Niu, Jianfeng.,Yang, Jiali.,Feng, Jianhua.,Feng, Zezhong.,Wang, Xia.,...&Wang, Guangce.(2022).Ubiquitin-proteasome pathway plays an essential regulatory role during spermatangium formation in Neopyropia yezoensis.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,62,12.
MLA Niu, Jianfeng,et al."Ubiquitin-proteasome pathway plays an essential regulatory role during spermatangium formation in Neopyropia yezoensis".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 62(2022):12.
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