Changes in gluconeogenesis pathways and key genes associated with mass mortality in the clam Meretrix petechialis upon Vibrio infection

doi:10.1016/j.aquaculture.2021.737691

IOCAS-IR > 实验海洋生物学重点实验室

	Changes in gluconeogenesis pathways and key genes associated with mass mortality in the clam Meretrix petechialis upon Vibrio infection
	Yu, Jiajia1,2 ; Wang, Hongxia 2; Liu, Baozhong2,3
	2022-02-15
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	548 页码:9
通讯作者	Liu, Baozhong(bzliu@qdio.ac.cn)
摘要	Mass mortality events frequently occur in marine bivalves growing in natural habitats and artificial culture environments due to pathogen infection. The clam Meretrix petechialis is an important commercial bivalve species, and the clam culture industry has suffered frequent mass mortality events during summer. However, the pathogenesis of the host is still poorly understood. In this study, M. petechialis was challenged with Vibrio parahaemolyticus, and the clams were sampled at different infection phases and physiological statuses. The transcriptome analysis of the clam hepatopancreas in the onset phase of Vibrio challenge showed the significant enrichment of multiple metabolism-related signaling pathways, including peroxisome proliferator-activated receptors (PPARs), adenosine 5'-monophosphate activated protein kinase (AMPK), phosphatidylinositol 3'-kinase (PI3K)-Akt signaling pathways and glycolysis/gluconeogenesis, which revealed the involvement of metabolism-related signaling pathways in host immune defense. Weighted correlation network analysis (WGCNA) revealed that key enzymes involved in gluconeogenesis were enriched in this phase, suggesting that gluconeogenesis may be associated with infection-induced mortality in clams. Examinations of the glucose content, phosphoenol-pyruvate carboxykinase (PEPCK) activity and the expression of key genes involved in gluconeogenesis showed that gluconeogenesis was repressed during Vibrio infection. An analysis of the expression of eight key genes in the PI3K-Akt signaling pathway between moribund and surviving individuals implied that gluconeogenesis was related to clam mass mortality. The results might help us to understand the molecular mechanism of death outbreak events and provide potential markers to detect the physiological status of this clam species.
关键词	Meretrix petechialis Vibrio Mass mortality Gluconeogenesis Marker
DOI	10.1016/j.aquaculture.2021.737691
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2019YFD0900700] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030304] ; China Agriculture Research System of MOF ; China Agriculture Research System of MARA
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000783848000011
出版者	ELSEVIER
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178841
专题	实验海洋生物学重点实验室
通讯作者	Liu, Baozhong
作者单位	1.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266000, Shandong, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Yu, Jiajia,Wang, Hongxia,Liu, Baozhong. Changes in gluconeogenesis pathways and key genes associated with mass mortality in the clam Meretrix petechialis upon Vibrio infection[J]. AQUACULTURE,2022,548:9.
APA	Yu, Jiajia,Wang, Hongxia,&Liu, Baozhong.(2022).Changes in gluconeogenesis pathways and key genes associated with mass mortality in the clam Meretrix petechialis upon Vibrio infection.AQUACULTURE,548,9.
MLA	Yu, Jiajia,et al."Changes in gluconeogenesis pathways and key genes associated with mass mortality in the clam Meretrix petechialis upon Vibrio infection".AQUACULTURE 548(2022):9.

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