Metabarcoding analysis of microbiome dynamics during a Phaeocystis globosa bloom in the Beibu Gulf, China

doi:10.1016/j.hal.2022.102217

	Metabarcoding analysis of microbiome dynamics during a Phaeocystis globosa bloom in the Beibu Gulf, China
	Gibson, Kate 1; Song, Huiyin 2,3,4; Chen, Nansheng 1,2,3,4
	2022-05-01
发表期刊	HARMFUL ALGAE
ISSN	1568-9883
卷号	114 页码:14
通讯作者	Chen, Nansheng(chenn@sfu.ca)
摘要	Phaeocystis globosa is an ecologically important haptophyte that can form harmful algal blooms (HABs). In this study, we used 16S rDNA V3-V4 amplicon sequencing data to explore the ecological mechanisms underlying a P. globosa bloom in the Beibu Gulf, China. Using field samples collected from three time points of a bloom, we observed a distinct succession in the bacteria, archaea and phytoplankton community composition throughout the bloom. We also observed temporal variation in response to the bloom at the nucleotide level, which supports a previously underappreciated amount of intragroup variation in the niches taken up by microbes during HABs. We developed a preliminary model for the development and progression of the P. globosa bloom using the spatial-temporal dynamics of P. globosa and the bacteria, archaea, phytoplankton and environmental variables. We also identified microbes with putative interactions with P. globosa during the bloom by identifying microbes corre-lated with P. globosa in interaction networks, identifying particle-associated microbes and exploring the P. globosa colony microbiome using sequences from whole P. globosa colonies collected during the bloom. This study revealed novel insight into the development of P. globosa HABs and many testable hypotheses that will guide future research on the mechanisms of P. globosa HABs.
关键词	Bloom Haptophyte 16S rDNA Amplicon Interaction Model
DOI	10.1016/j.hal.2022.102217
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2017YFC1404302] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000] ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Labora-tory for Marine Science and Technology[LMEES-CTSP-2018-1] ; Chinese Academy of Sciences Pioneer Hundred Talents Program ; Taishan Scholar Project Special Fund ; Qingdao Innovation and Creation Plan alent Development Program-5th Annual Pioneer and Innovator Leadership Award )[19-3-2-16-zhc] ; Youth Talent Support Program of the Laboratory for Marine Ecology and Environmental Science ; Pilot Na-tional Laboratory for Marine Science and Technology (Qingdao)[LMEES-YTSP-2018-01-08]
WOS研究方向	Marine & Freshwater Biology
WOS类目	Marine & Freshwater Biology
WOS记录号	WOS:000782314500001
出版者	ELSEVIER
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178808
专题	海洋生态与环境科学重点实验室
通讯作者	Chen, Nansheng
作者单位	1.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Gibson, Kate,Song, Huiyin,Chen, Nansheng. Metabarcoding analysis of microbiome dynamics during a Phaeocystis globosa bloom in the Beibu Gulf, China[J]. HARMFUL ALGAE,2022,114:14.
APA	Gibson, Kate,Song, Huiyin,&Chen, Nansheng.(2022).Metabarcoding analysis of microbiome dynamics during a Phaeocystis globosa bloom in the Beibu Gulf, China.HARMFUL ALGAE,114,14.
MLA	Gibson, Kate,et al."Metabarcoding analysis of microbiome dynamics during a Phaeocystis globosa bloom in the Beibu Gulf, China".HARMFUL ALGAE 114(2022):14.

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