<p>Use of quantitative real-time PCR to select reference genes in the testis and ovary of Apostichopus japonicus during the breeding period</p>

doi:10.1016/j.aqrep.2022.101010

	Use of quantitative real-time PCR to select reference genes in the testis and ovary of Apostichopus japonicus during the breeding period
	Zhang, Shuangyan 1,2,3,4,5,6; Ru, Xiaoshang 1,2,3,4,6; Su, Fang 1,2,3,4,5,6; Liang, Wenke 1,2,3,4,6; Zhang, Libin 1,2,3,4,5,6; Yang, Hongsheng1,2,3,4,5,6,7
	2022-02-01
发表期刊	AQUACULTURE REPORTS
ISSN	2352-5134
卷号	22 页码:7
通讯作者	Zhang, Libin(zhanglibin@qdio.ac.cn)
摘要	Gonads play a critical role in the reproduction of sea cucumbers, and their development is regulated by a large number of genes. To date, the ideal reference genes (RGs) for studying sea cucumber gonadal development have not been reported. In this study, we selected ten RGs and conducted quantitative real-time PCR analysis to identify the most stable among Apostichopus japonicus gonads at four different stages of gonadal development. We used delta C-q, GeNorm, NormFinder, BestKeeper, and RefFinder to evaluate the stability of candidate genes. EF1 beta and Grb2 together were the preferred combination of stable RGs among all of the gonad samples and for the ovaries alone. UBQ combined with RPS4 was the best combination of stable RGs in the testes. This study aimed to provide a basis for analysis of differences in gene expression related to gonadal development in sea cucumbers, and the results provide valuable information for further studies of the molecular mechanisms involved in sea cucumber gonadal development.
关键词	Expression stability Gonads Quantitative real-time PCR Reference genes Sea cucumber breeding period
DOI	10.1016/j.aqrep.2022.101010
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[42061160365] ; National Natural Science Foundation of China[41876157] ; Scientific Research and Tech-nological Development Program of Fangchenggang[AB20014021] ; International Partners Program of the Chinese Academy of Sciences[133137KYSB20180069]
WOS研究方向	Fisheries
WOS类目	Fisheries
WOS记录号	WOS:000780442200001
出版者	ELSEVIER
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178742
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Libin
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 7.Chinese Acad Sci, Innovat Seed Design, Wuhan 430000, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心; 中国科学院海洋研究所
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心; 中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Shuangyan,Ru, Xiaoshang,Su, Fang,et al. Use of quantitative real-time PCR to select reference genes in the testis and ovary of Apostichopus japonicus during the breeding period [J]. AQUACULTURE REPORTS,2022,22:7.
APA	Zhang, Shuangyan,Ru, Xiaoshang,Su, Fang,Liang, Wenke,Zhang, Libin,&Yang, Hongsheng.(2022). Use of quantitative real-time PCR to select reference genes in the testis and ovary of Apostichopus japonicus during the breeding period .AQUACULTURE REPORTS,22,7.
MLA	Zhang, Shuangyan,et al." Use of quantitative real-time PCR to select reference genes in the testis and ovary of Apostichopus japonicus during the breeding period ".AQUACULTURE REPORTS 22(2022):7.

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