Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
Use of quantitative real-time PCR to select reference genes in the testis and ovary of Apostichopus japonicus during the breeding period | |
Zhang, Shuangyan1,2,3,4,5,6; Ru, Xiaoshang1,2,3,4,6; Su, Fang1,2,3,4,5,6; Liang, Wenke1,2,3,4,6; Zhang, Libin1,2,3,4,5,6; Yang, Hongsheng1,2,3,4,5,6,7
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| 2022-02-01 | |
| Source Publication | AQUACULTURE REPORTS
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| ISSN | 2352-5134 |
| Volume | 22Pages:7 |
| Corresponding Author | Zhang, Libin(zhanglibin@qdio.ac.cn) |
| Abstract | Gonads play a critical role in the reproduction of sea cucumbers, and their development is regulated by a large number of genes. To date, the ideal reference genes (RGs) for studying sea cucumber gonadal development have not been reported. In this study, we selected ten RGs and conducted quantitative real-time PCR analysis to identify the most stable among Apostichopus japonicus gonads at four different stages of gonadal development. We used delta C-q, GeNorm, NormFinder, BestKeeper, and RefFinder to evaluate the stability of candidate genes. EF1 beta and Grb2 together were the preferred combination of stable RGs among all of the gonad samples and for the ovaries alone. UBQ combined with RPS4 was the best combination of stable RGs in the testes. This study aimed to provide a basis for analysis of differences in gene expression related to gonadal development in sea cucumbers, and the results provide valuable information for further studies of the molecular mechanisms involved in sea cucumber gonadal development. |
| Keyword | Expression stability Gonads Quantitative real-time PCR Reference genes Sea cucumber breeding period |
| DOI | 10.1016/j.aqrep.2022.101010 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[42061160365] ; National Natural Science Foundation of China[41876157] ; Scientific Research and Tech-nological Development Program of Fangchenggang[AB20014021] ; International Partners Program of the Chinese Academy of Sciences[133137KYSB20180069] |
| WOS Research Area | Fisheries |
| WOS Subject | Fisheries |
| WOS ID | WOS:000780442200001 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/178742 |
| Collection | 海洋生态与环境科学重点实验室 |
| Corresponding Author | Zhang, Libin |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 7.Chinese Acad Sci, Innovat Seed Design, Wuhan 430000, Peoples R China |
| First Author Affilication | Key Laboratory of Marine Ecology & Environmental Sciences, CAS; Center for Ocean Mega-Science, Chinese Academy of Sciences; Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Key Laboratory of Marine Ecology & Environmental Sciences, CAS; Center for Ocean Mega-Science, Chinese Academy of Sciences; Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 |
Zhang, Shuangyan,Ru, Xiaoshang,Su, Fang,et al. Use of quantitative real-time PCR to select reference genes in the testis and ovary of Apostichopus japonicus during the breeding period [J]. AQUACULTURE REPORTS,2022,22:7. |
| APA |
Zhang, Shuangyan,Ru, Xiaoshang,Su, Fang,Liang, Wenke,Zhang, Libin,&Yang, Hongsheng.(2022). Use of quantitative real-time PCR to select reference genes in the testis and ovary of Apostichopus japonicus during the breeding period .AQUACULTURE REPORTS,22,7. |
| MLA |
Zhang, Shuangyan,et al." Use of quantitative real-time PCR to select reference genes in the testis and ovary of Apostichopus japonicus during the breeding period ".AQUACULTURE REPORTS 22(2022):7. |
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| File Name/Size | DocType | Version | Access | License | ||
| 1-s2.0-S235251342200（1835KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | View | |
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