Divergences of the RLR Gene Families across Lophotrochozoans: Domain Grafting, Exon-Intron Structure, Expression, and Positive Selection

doi:10.3390/ijms23073415

IOCAS-IR > 实验海洋生物学重点实验室

	Divergences of the RLR Gene Families across Lophotrochozoans: Domain Grafting, Exon-Intron Structure, Expression, and Positive Selection
	Yao, Shanshan 1,2,3,4,5; Chan, Jiulin 2,3,4,5; Xu, Yue 2,3,4,5; Wu, Shimei 1; Zhang, Linlin 2,3,4,5,6
	2022-04-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	23 期号:7 页码:20
通讯作者	Zhang, Linlin(linlinzhang@qdio.ac.cn)
摘要	Invertebrates do not possess adaptive immunity but have evolved a variety of unique repertoires of innate immune sensors. In this study, we explored the immune diversity and specificity of invertebrates based on the lophotrochozoan RLRs, a major component in antiviral immune recognition. By annotating RLRs in the genomes of 58 representative species across metazoan evolution, we explored the gene expansion of RLRs in Lophotrochozoa. Of note, the N-terminal domains of lophotrochozoan RLRs showed the most striking diversity which evolved independently by domain grafting. Exon-intron structures were revealed to be prevalent in the domain grafting of lophotrochozoan RLRs based on an analysis of sibling paralogs and orthologs. In more than half of the cases, the mechanism of 'exonization/pseudoexonization' led to the generation of non-canonical N-terminal domains. Transcriptomic studies revealed that many non-canonical RLRs display immune-related expression patterns. Two of these RLRs showed obvious evidence of positive selection, which may be the result of host defense selection pressure. Overall, our study suggests that the complex and unique domain arrangement of lophotrochozoan RLRs might result from domain grafting, exon-intron divergence, expression diversification, and positive selection, which may have led to functionally distinct lophotrochozoan RLRs.
关键词	RIG-I-like receptor lophotrochozoan innate immune molecular evolution gene expression domain grafting exon-intron structure positive selection
DOI	10.3390/ijms23073415
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41976088] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; Key Development Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Science[COMS2019R01]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000781023800001
出版者	MDPI
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178712
专题	实验海洋生物学重点实验室深海极端环境与生命过程研究中心
通讯作者	Zhang, Linlin
作者单位	1.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, CAS, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, Ctr Deep Sea Res, Qingdao 266071, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 6.Univ Chinese Acad Sci, Coll Marine Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Yao, Shanshan,Chan, Jiulin,Xu, Yue,et al. Divergences of the RLR Gene Families across Lophotrochozoans: Domain Grafting, Exon-Intron Structure, Expression, and Positive Selection[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(7):20.
APA	Yao, Shanshan,Chan, Jiulin,Xu, Yue,Wu, Shimei,&Zhang, Linlin.(2022).Divergences of the RLR Gene Families across Lophotrochozoans: Domain Grafting, Exon-Intron Structure, Expression, and Positive Selection.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(7),20.
MLA	Yao, Shanshan,et al."Divergences of the RLR Gene Families across Lophotrochozoans: Domain Grafting, Exon-Intron Structure, Expression, and Positive Selection".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.7(2022):20.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
ijms-23-03415.pdf（14892KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览