IOCAS-IR
Highly Sensitive and Specific Responses of Oyster Hemocytes to Copper Exposure: Single-Cell Transcriptomic Analysis of Different Cell Populations
Meng, Jie1,2,3; Wang, Wen-Xiong1,4,5,6
2022-02-15
发表期刊ENVIRONMENTAL SCIENCE & TECHNOLOGY
ISSN0013-936X
卷号56期号:4页码:2497-2510
通讯作者Wang, Wen-Xiong(wx.wang@cityu.edu.hk)
摘要Oyster hemocytes are the primary vehicles transporting and detoxifying metals and are regarded as important cells for the occurrence of colored oysters due to copper (Cu) contamination. However, its heterogeneous responses under Cu exposure have not been studied. Single-cell transcriptome profiling (scRNA-seq) provides high-resolution visual insights into tissue dynamics and environmental responses. Here, we used scRNA-seq to study the responses of different cell populations of hemocytes under Cu exposure in an estuarine oyster Crassostrea hongkongensis. The 1900 population-specific Cu-responsive genes were identified in 12 clusters of hemocytes, which provided a more sensitive technique for examining Cu exposure. The granulocyte, semi-granulocyte, and hyalinocyte had specific responses, while the granulocyte was the most important responsive cell type and displayed heterogeneity responses of its two subtypes. In one subtype, Cu was transported with metal transporters and chelated with Cu chaperons in the cytoplasm. Excess Cu disturbed oxidative phosphorylation and induced reactive oxygen species production. However, in the other subtype, endocytosis was mainly responsible for Cu internalization, which was sequestered in membrane-bound granules. Collectively, our results provided the first mRNA expression profile of hemocytes in oysters and revealed the heterogeneity responses under Cu exposure.
关键词oyster Cu exposure scRNA-seq granulocytes heterogeneity responses
DOI10.1021/acs.est.1c07510
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[32102815] ; Youth Innovation Promotion Association of the Chinese Academy of Science[2019210]
WOS研究方向Engineering ; Environmental Sciences & Ecology
WOS类目Engineering, Environmental ; Environmental Sciences
WOS记录号WOS:000763099700040
出版者AMER CHEMICAL SOC
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/178438
专题中国科学院海洋研究所
通讯作者Wang, Wen-Xiong
作者单位1.Southern Marine Sci & Engn Guangdong Lab Guangzho, Guangzhou 511458, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
4.City Univ Hong Kong, Sch Energy & Environm, Hong Kong 999077, Peoples R China
5.City Univ Hong Kong, State Key Lab Marine Pollut, Hong Kong 999077, Peoples R China
6.City Univ Hong Kong, Res Ctr Oceans & Human Hlth, Shenzhen Res Inst, Shenzhen 518057, Peoples R China
第一作者单位实验海洋生物学重点实验室
推荐引用方式
GB/T 7714
Meng, Jie,Wang, Wen-Xiong. Highly Sensitive and Specific Responses of Oyster Hemocytes to Copper Exposure: Single-Cell Transcriptomic Analysis of Different Cell Populations[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2022,56(4):2497-2510.
APA Meng, Jie,&Wang, Wen-Xiong.(2022).Highly Sensitive and Specific Responses of Oyster Hemocytes to Copper Exposure: Single-Cell Transcriptomic Analysis of Different Cell Populations.ENVIRONMENTAL SCIENCE & TECHNOLOGY,56(4),2497-2510.
MLA Meng, Jie,et al."Highly Sensitive and Specific Responses of Oyster Hemocytes to Copper Exposure: Single-Cell Transcriptomic Analysis of Different Cell Populations".ENVIRONMENTAL SCIENCE & TECHNOLOGY 56.4(2022):2497-2510.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
acs.est.1c07510.pdf(9332KB)期刊论文出版稿限制开放CC BY-NC-SA浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Meng, Jie]的文章
[Wang, Wen-Xiong]的文章
百度学术
百度学术中相似的文章
[Meng, Jie]的文章
[Wang, Wen-Xiong]的文章
必应学术
必应学术中相似的文章
[Meng, Jie]的文章
[Wang, Wen-Xiong]的文章
相关权益政策
暂无数据
收藏/分享
文件名: acs.est.1c07510.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。