Molecular Identification of Species Diversity Using Pelagic Fish Eggs in Spring and Late Autumn-Winter in the Eastern Beibu Gulf, China

doi:10.3389/fmars.2021.806208

	Molecular Identification of Species Diversity Using Pelagic Fish Eggs in Spring and Late Autumn-Winter in the Eastern Beibu Gulf, China
	Hou, Gang 1; Chen, Yanying 1; Wang, Jinrun 1; Pan, Chuanhao 1; Lin, Jianbin 1; Feng, Bo 1; Zhang, Hui2,3,4
	2022-01-28
发表期刊	FRONTIERS IN MARINE SCIENCE
卷号	8 页码:13
通讯作者	Feng, Bo(fengb@gdou.edu.cn) ; Zhang, Hui(zhanghui@qdio.ac.cn)
摘要	The Beibu Gulf is considered as one of China's four major fishing grounds, although the substantial overexploitation of fisheries has led to the collapse of many fish stocks, and to changes to spawning grounds in recent decades. Classifying fish eggs is an important way to monitor the recruitment process and identify the spawning sites of fish. However, the lack of a basis for morphological identification and difficulties in correctly identifying fish eggs based on morphological characteristics has limited scientific studies. In the present study, we identified fish eggs using molecular detection of cytochrome c oxidase subunit I and cytochrome b fragments. Ichthyoplankton surveys were conducted in the spring and late autumn-winter of 2020 in the eastern Beibu Gulf. Among the DNA extracted from the 873 chosen fish eggs, we successfully obtained 541 high-quality cytochrome c oxidase subunit I sequences and 41 high-quality cytochrome b sequences. We successfully identified 212 fish eggs (36.4%) from 32 species; 291 eggs (50.0%) showed ambiguous species delimitation, and 79 eggs (13.6%) could not be identified. Among the identified species, we found 25 species in spring and 25 species in late autumn-winter, out of which 18 species occurred in both seasons. We also obtained high resolution photographs of fish eggs at the species level for further morphological analysis and identification. The present study confirms the efficacy of using molecular methods to identify fish species from eggs and provides valuable information for protecting the spawning ground of economically valuable fish and for managing fishery resources.
关键词	fish eggs molecular species identification spawning site fish conservation Beibu Gulf
DOI	10.3389/fmars.2021.806208
收录类别	SCI
语种	英语
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000760602200001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178222
专题	海洋生态与环境科学重点实验室
通讯作者	Feng, Bo; Zhang, Hui
作者单位	1.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Hou, Gang,Chen, Yanying,Wang, Jinrun,et al. Molecular Identification of Species Diversity Using Pelagic Fish Eggs in Spring and Late Autumn-Winter in the Eastern Beibu Gulf, China[J]. FRONTIERS IN MARINE SCIENCE,2022,8:13.
APA	Hou, Gang.,Chen, Yanying.,Wang, Jinrun.,Pan, Chuanhao.,Lin, Jianbin.,...&Zhang, Hui.(2022).Molecular Identification of Species Diversity Using Pelagic Fish Eggs in Spring and Late Autumn-Winter in the Eastern Beibu Gulf, China.FRONTIERS IN MARINE SCIENCE,8,13.
MLA	Hou, Gang,et al."Molecular Identification of Species Diversity Using Pelagic Fish Eggs in Spring and Late Autumn-Winter in the Eastern Beibu Gulf, China".FRONTIERS IN MARINE SCIENCE 8(2022):13.

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