Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands

doi:10.3390/microorganisms10020476

IOCAS-IR > 实验海洋生物学重点实验室

	Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands
	Liu, Xiaojie1,2,3 ; Zhao, Jin 1,2; Jiang, Peng 1,2
	2022-02-01
发表期刊	MICROORGANISMS
卷号	10 期号:2 页码:8
通讯作者	Jiang, Peng(jiangpeng@qdio.ac.cn)
摘要	Macroalgae-associated bacteria play an important role in their algal hosts' biological processes. They are localized on surfaces of the host thalli, as well as between and even within algal cells. To examine the differences in community structures and functions between epi- and endo- bacteria, an effective approach for maximizing epiphyte removal from delicate seaweeds while retaining endophyte fidelity must be developed. In this study, a variety of surface sterilization methods for Ulva prolifera were compared, including mechanical, chemical, and enzymatical treatments. According to the results of scanning electron microscope (SEM) and denaturing gradient gel electrophoresis (DGGE) analysis, almost complete removal of epiphytic bacteria on Ulva was obtained simply by co-vortex of seaweeds with silica sands, causing minimal disturbance to endosymbionts when compared to previous published methods. In addition, the adaptability was also confirmed in additional U. prolifera strains and Ulva species with blade-like or narrow tubular thallus shapes. This easy mechanical method would enable the analysis of community composition and host specificity for Ulva-associated epi- and endo-bacteria separately.
关键词	associated bacteria seaweed silica sand surface sterilization Ulva
DOI	10.3390/microorganisms10020476
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41776153] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Science[COMS2019Q05] ; Key R&D Program of Shandong Province[2019GSF107012] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050302] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42030302]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000764421500001
出版者	MDPI
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178180
专题	实验海洋生物学重点实验室
通讯作者	Jiang, Peng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Xiaojie,Zhao, Jin,Jiang, Peng. Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands[J]. MICROORGANISMS,2022,10(2):8.
APA	Liu, Xiaojie,Zhao, Jin,&Jiang, Peng.(2022).Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands.MICROORGANISMS,10(2),8.
MLA	Liu, Xiaojie,et al."Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands".MICROORGANISMS 10.2(2022):8.

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