Nonmuscle Myosin II is Required for Larval Shell Formation in a Patellogastropod

doi:10.3389/fcell.2022.813741

IOCAS-IR > 实验海洋生物学重点实验室

	Nonmuscle Myosin II is Required for Larval Shell Formation in a Patellogastropod
	Liu, Xinyu 1,2,3,4; Huan, Pin 1,2,3; Liu, Baozhong1,2,3,4
	2022-02-03
发表期刊	FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
ISSN	2296-634X
卷号	10 页码:11
通讯作者	Liu, Baozhong(bzliu@qdio.ac.cn)
摘要	The molecular mechanisms underlying larval shell development in mollusks remain largely elusive. We previously found evident filamentous actin (F-actin) aggregations in the developing shell field of the patellogastropod Lottia goshimai, indicating roles of actomyosin networks in the process. In the present study, we functionally characterized nonmuscle myosin II (NM II), the key molecule in actomyosin networks, in the larval shell development of L. goshimai. Immunostaining revealed general colocalization of phosphorylated NM II and F-actin in the shell field. When inhibiting the phosphorylation of NM II using the specific inhibitor blebbistatin in one- or 2-h periods during shell field morphogenesis (6-8 h post-fertilization, hpf), the larval shell plate was completely lost in the veliger larva (24 hpf). Scanning electron microscopy revealed that the nascent larval shell plate could not be developed in the manipulated larvae (10 hpf). Further investigations revealed that key events in shell field morphogenesis were inhibited by blebbistatin pulses, including invagination of the shell field and cell shape changes and cell rearrangements during shell field morphogenesis. These factors caused the changed morphology of the shell field, despite the roughly retained "rosette" organization. To explore whether the specification of related cells was affected by blebbistatin treatments, we investigated the expression of four potential shell formation genes (bmp2/4, gata2/3, hox1 and engrailed). The four genes did not show evident changes in expression level, indicating unaffected cell specification in the shell field, while the gene expression patterns showed variations according to the altered morphology of the shell field. Together, our results reveal that NM II contributes to the morphogenesis of the shell field and is crucial for the formation of the larval shell plate in L. goshimai. These results add to the knowledge of the mechanisms of molluskan shell development.
关键词	Lottia goshimai shell actomyosin F-actin nonmuscle myosin II
DOI	10.3389/fcell.2022.813741
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41776157] ; National Natural Science Foundation of China[42076123] ; National Key R&D Program of China[2018YFD0900104] ; China Agriculture Research System[CARS-49] ; Natural Science Foundation of Shandong Province[ZR2016CQ30] ; Youth Innovation Promotion Association CAS[2018239] ; Taishan Scholar Project Fund of Shandong Province of China[tsqn202103129]
WOS研究方向	Cell Biology ; Developmental Biology
WOS类目	Cell Biology ; Developmental Biology
WOS记录号	WOS:000759811600001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178135
专题	实验海洋生物学重点实验室
通讯作者	Liu, Baozhong
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Liu, Xinyu,Huan, Pin,Liu, Baozhong. Nonmuscle Myosin II is Required for Larval Shell Formation in a Patellogastropod[J]. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,2022,10:11.
APA	Liu, Xinyu,Huan, Pin,&Liu, Baozhong.(2022).Nonmuscle Myosin II is Required for Larval Shell Formation in a Patellogastropod.FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,10,11.
MLA	Liu, Xinyu,et al."Nonmuscle Myosin II is Required for Larval Shell Formation in a Patellogastropod".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 10(2022):11.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
fcell-10-813741.pdf（2585KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览