IOCAS-IR
Microfluidic-based in vitro thrombosis model for studying microplastics toxicity
Chen, Longfei1,2; Zheng, Yajing3; Liu, Yantong1,2; Tian, Pengfu1; Yu, Le1; Bai, Long4; Zhou, Fuling5; Yang, Yi1,2; Cheng, Yanxiang3; Wang, Fubing6; Zheng, Li7; Jiang, Fenghua7; Zhu, Yimin4
2022-02-09
Source PublicationLAB ON A CHIP
ISSN1473-0197
Pages10
Corresponding AuthorYang, Yi(yangyiys@whu.edu.cn) ; Cheng, Yanxiang(yanxiangCheng@whu.edu.cn)
AbstractThe potential impact of microplastics (MPs) on health has caused great concern, and a toxicology platform that realistically reproduces the system behaviour is urgently needed to further explore and validate MP-related health issues. Herein, we introduce an optically assisted thrombus platform to reveal the interaction of MPs with the vascular system. The risk of accumulation has also been evaluated using a mouse model, and the effect of MPs on the properties of the thrombus are validated via in vitro experiments. The microfluidic system is endothelialized, and the regional tissue injury-induced thrombosis is then realized through optical irradiation. Whole blood is perfused with MPs, and the invasion process visualized and recorded. The mouse model shows a cumulative risk in the blood with continuous exposure to MPs (P-value < 0.0001). The on-chip results show that MP invasion leads to decreased binding of fibrin to platelets (P-value < 0.0001), which is consistent with the results of the in vitro experiments, and shows a high risk of thrombus shedding in real blood flow compared with normal thrombus. This work provides a new method to further reveal MP-related health risks.
DOI10.1039/d1lc00989c
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFC1003200] ; National Natural Science Foundation of China[62175190] ; National Natural Science Foundation of China[11774274] ; National Natural Science Foundation of China[81860276] ; National Natural Science Foundation of China[81770179] ; Foundation Research Fund of Shenzhen Science and Technology Program[JCYJ20190808154409678] ; Support Projects of Medical Science and Technology Innovation Platform of Wuhan University Zhongnan Hospital in 2020[lcyf202010]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation
WOS IDWOS:000757486500001
PublisherROYAL SOC CHEMISTRY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/178057
Collection中国科学院海洋研究所
Corresponding AuthorYang, Yi; Cheng, Yanxiang
Affiliation1.Wuhan Univ, Sch Phys & Technol, Key Lab Artificial Micro & Nanostruct, Minist Educ, Wuhan 430072, Peoples R China
2.Wuhan Univ, Shenzhen Res Inst, Shenzhen 518000, Peoples R China
3.Wuhan Univ, Dept Obstet & Gynecol, Renmin Hosp, Wuhan 430060, Peoples R China
4.Zhejiang Univ, Sch Med, Hangzhou 310002, Zhejiang, Peoples R China
5.Wuhan Univ, Zhongnan Hosp, Dept Hematol, Wuhan 430071, Peoples R China
6.Wuhan Univ, Zhongnan Hosp, Dept Lab Med, Wuhan 430071, Peoples R China
7.Chinese Acad Sci, Inst Oceanol, Qingdao 266061, Peoples R China
Recommended Citation
GB/T 7714
Chen, Longfei,Zheng, Yajing,Liu, Yantong,et al. Microfluidic-based in vitro thrombosis model for studying microplastics toxicity[J]. LAB ON A CHIP,2022:10.
APA Chen, Longfei.,Zheng, Yajing.,Liu, Yantong.,Tian, Pengfu.,Yu, Le.,...&Zhu, Yimin.(2022).Microfluidic-based in vitro thrombosis model for studying microplastics toxicity.LAB ON A CHIP,10.
MLA Chen, Longfei,et al."Microfluidic-based in vitro thrombosis model for studying microplastics toxicity".LAB ON A CHIP (2022):10.
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