Zeolitic Imidazolate Framework-8 Grown on Nanoporous SiO2 with Aptamer Functionalization for Selective Isolation of Thrombin
Lin, Yanna1; Xu, Yulong1; Xing, Yanzhi1; Liu, Nazhen2; Chen, Xuwei1
2021-11-26
发表期刊ACS APPLIED NANO MATERIALS
卷号4期号:11页码:12204-12212
通讯作者Liu, Nazhen(liunazhen@qdio.ac.cn) ; Chen, Xuwei(chenxuwei@mail.neu.edu.cr)
摘要A three-dimensional ordered macroporous composite (3DOM SiO2@ZIF-8-TBA) was fabricated via the in situ growth of metal-organic framework ZIF-8 (similar to 50 nm) on the surface of macroporous SiO2 (pore size of similar to 250 nm) and the subsequent functionalization with a thrombin aptamer. The obtained 3DOM SiO2@ZIF-8-TBA composite has been proven to be a potential protein-enriching adsorbent, which exhibits high selectivity toward the adsorption of thrombin due to the specific conformation between the aptamer and thrombin. The adsorption behavior of thrombin onto the 3DOM SiO2@ZIF-8-TBA composite conforms to the Langmuir adsorption model, resulting in a superior adsorption capacity of 40.9 U mg(-1), contributed by the ordered macroporous structure. The retained thrombin could be readily recovered by 1.0% NH3 center dot H2O (m/v) with a recovery of 92.3%. Circular dichroism spectra illustrate that the 3DOM SiO2@ZIF-8-TBA composite-based adsorption/desorption processes have no influence on thrombin conformation. The application of the 3DOM SiO2@ZIF-8-TBA composite in separating thrombin from human serum has been verified; fibrin production tests prove that the activity of recovered thrombin is well retained.
关键词3D-ordered macroporous structure zeolitic imidazolate framework-8 nanoporous SiO2 solid-phase extraction aptamer thrombin
DOI10.1021/acsanm.1c02691
收录类别SCI
语种英语
资助项目Natural Science Foundation of China[21727811] ; Fundamental Research Funds for the Central Universities[N2005027]
WOS研究方向Science & Technology - Other Topics ; Materials Science
WOS类目Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号WOS:000752894100083
出版者AMER CHEMICAL SOC
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/178006
专题海洋环境腐蚀与生物污损重点实验室
通讯作者Liu, Nazhen; Chen, Xuwei
作者单位1.Northeastern Univ, Coll Sci, Res Ctr Analyt Sci, Dept Chem, Shenyang 110819, Peoples R China
2.Chinese Acad Sci, Key Lab Marine Environm Corros & Biofouling, Inst Oceanol, Qingdao 266071, Peoples R China
通讯作者单位中国科学院海洋研究所
推荐引用方式
GB/T 7714
Lin, Yanna,Xu, Yulong,Xing, Yanzhi,et al. Zeolitic Imidazolate Framework-8 Grown on Nanoporous SiO2 with Aptamer Functionalization for Selective Isolation of Thrombin[J]. ACS APPLIED NANO MATERIALS,2021,4(11):12204-12212.
APA Lin, Yanna,Xu, Yulong,Xing, Yanzhi,Liu, Nazhen,&Chen, Xuwei.(2021).Zeolitic Imidazolate Framework-8 Grown on Nanoporous SiO2 with Aptamer Functionalization for Selective Isolation of Thrombin.ACS APPLIED NANO MATERIALS,4(11),12204-12212.
MLA Lin, Yanna,et al."Zeolitic Imidazolate Framework-8 Grown on Nanoporous SiO2 with Aptamer Functionalization for Selective Isolation of Thrombin".ACS APPLIED NANO MATERIALS 4.11(2021):12204-12212.
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