IOCAS-IR  > 海洋生态与环境科学重点实验室
Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments
Liu, Wang1,2; Zheng, Xiaowei1; Dai, Xin1,2; Zhang, Zhenfeng1; Zhang, Wenyan3,4,5; Xiao, Tian3,4,5; Huang, Li1,2
2022
Source PublicationVIRUSES-BASEL
Volume14Issue:1Pages:14
Corresponding AuthorHuang, Li(huangl@im.ac.cn)
AbstractViruses are far more abundant than cellular microorganisms in the marine ecosystem. However, very few viruses have so far been isolated from marine sediments, especially hydrothermal vent sediments, hindering the understanding of the biology and ecological functions of these tiny organisms. Here, we report the isolation and characterization of a temperate bacteriophage, named PVJ1, which infects Psychrobacillus from a hydrothermal vent field in Okinawa Trough. PVJ1 belongs to the Myoviridae family of the order Caudovirales. The tailed phage possesses a 53,187 bp linear dsDNA genome, with 84 ORFs encoding structural proteins, genome replication, host lysis, etc. in a modular pattern. The phage genome is integrated into the host chromosome near the 3 '-end of deoD, a gene encoding purine nucleoside phosphorylase (PNP). The phage integration does not appear to disrupt the function of PNP. The phage DNA is packaged by the headful mechanism. Release of PVJ1 from the host cell was drastically enhanced by treatment with mitomycin C. Phages encoding an MCP sharing significant similarity (>= 70% identical amino acids) with that of PVJ1 are widespread in diverse environments, including marine and freshwater sediments, soils, artificial ecosystems, and animal intestines, and primarily infect Firmicutes. These results are valuable to the understanding of the lifestyle and host interactions of bacterial viruses at the bottom of the ocean.
KeywordPsychrobacillus bacteriophage marine sediments Myoviridae
DOI10.3390/v14010108
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFC0310703] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; China Ocean Mineral Resources RD Association[DY135-B2-02] ; National Natural Science Foundation of China[31970170] ; National Natural Science Foundation of China[92051109] ; National Natural Science Foundation of China[92151001] ; National Natural Science Foundation of China[KEX-UE2019GZ05] ; National Natural Science Foundation of China[KEXUE2019GZ03]
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000747457100001
PublisherMDPI
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/177712
Collection海洋生态与环境科学重点实验室
Corresponding AuthorHuang, Li
Affiliation1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266273, Peoples R China
Recommended Citation
GB/T 7714
Liu, Wang,Zheng, Xiaowei,Dai, Xin,et al. Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments[J]. VIRUSES-BASEL,2022,14(1):14.
APA Liu, Wang.,Zheng, Xiaowei.,Dai, Xin.,Zhang, Zhenfeng.,Zhang, Wenyan.,...&Huang, Li.(2022).Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments.VIRUSES-BASEL,14(1),14.
MLA Liu, Wang,et al."Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments".VIRUSES-BASEL 14.1(2022):14.
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