Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments

doi:10.3390/v14010108

	Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments
	Liu, Wang 1,2; Zheng, Xiaowei 1; Dai, Xin 1,2; Zhang, Zhenfeng 1; Zhang, Wenyan 3,4,5; Xiao, Tian 3,4,5; Huang, Li 1,2
	2022
发表期刊	VIRUSES-BASEL
卷号	14 期号:1 页码:14
通讯作者	Huang, Li(huangl@im.ac.cn)
摘要	Viruses are far more abundant than cellular microorganisms in the marine ecosystem. However, very few viruses have so far been isolated from marine sediments, especially hydrothermal vent sediments, hindering the understanding of the biology and ecological functions of these tiny organisms. Here, we report the isolation and characterization of a temperate bacteriophage, named PVJ1, which infects Psychrobacillus from a hydrothermal vent field in Okinawa Trough. PVJ1 belongs to the Myoviridae family of the order Caudovirales. The tailed phage possesses a 53,187 bp linear dsDNA genome, with 84 ORFs encoding structural proteins, genome replication, host lysis, etc. in a modular pattern. The phage genome is integrated into the host chromosome near the 3 '-end of deoD, a gene encoding purine nucleoside phosphorylase (PNP). The phage integration does not appear to disrupt the function of PNP. The phage DNA is packaged by the headful mechanism. Release of PVJ1 from the host cell was drastically enhanced by treatment with mitomycin C. Phages encoding an MCP sharing significant similarity (>= 70% identical amino acids) with that of PVJ1 are widespread in diverse environments, including marine and freshwater sediments, soils, artificial ecosystems, and animal intestines, and primarily infect Firmicutes. These results are valuable to the understanding of the lifestyle and host interactions of bacterial viruses at the bottom of the ocean.
关键词	Psychrobacillus bacteriophage marine sediments Myoviridae
DOI	10.3390/v14010108
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFC0310703] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; China Ocean Mineral Resources RD Association[DY135-B2-02] ; National Natural Science Foundation of China[31970170] ; National Natural Science Foundation of China[92051109] ; National Natural Science Foundation of China[92151001] ; National Natural Science Foundation of China[KEX-UE2019GZ05] ; National Natural Science Foundation of China[KEXUE2019GZ03]
WOS研究方向	Virology
WOS类目	Virology
WOS记录号	WOS:000747457100001
出版者	MDPI
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177712
专题	海洋生态与环境科学重点实验室
通讯作者	Huang, Li
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266273, Peoples R China
推荐引用方式 GB/T 7714	Liu, Wang,Zheng, Xiaowei,Dai, Xin,et al. Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments[J]. VIRUSES-BASEL,2022,14(1):14.
APA	Liu, Wang.,Zheng, Xiaowei.,Dai, Xin.,Zhang, Zhenfeng.,Zhang, Wenyan.,...&Huang, Li.(2022).Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments.VIRUSES-BASEL,14(1),14.
MLA	Liu, Wang,et al."Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments".VIRUSES-BASEL 14.1(2022):14.

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viruses-14-00108.pdf（3279KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览