Development of sensitive genotype-specific quantitative polymerase chain reaction methods for detection of Phaeocystis globosa in the South China Sea

doi:10.1002/lom3.10476

	Development of sensitive genotype-specific quantitative polymerase chain reaction methods for detection of Phaeocystis globosa in the South China Sea
	Niu, Zhuang 1,2,3,4; Liu, Chao 1,2,3,4; Zhang, Qing-Chun1,2,4 ; Wang, Jin-Xiu 1,2,3,4; Kong, Fan-Zhou1,2,4 ; Hu, Xiao-Kun 1,2,3,4; Yu, Ren-Cheng 1,2,3,4
	2021-12-27
发表期刊	LIMNOLOGY AND OCEANOGRAPHY-METHODS
ISSN	1541-5856
页码	15
通讯作者	Zhang, Qing-Chun(qczhang@qdio.ac.cn)
摘要	Since 1997, dense blooms of the alga Phaeocystis globosa have occurred in the South China Sea (SCS), and previous studies have revealed the co-existence in this sea of two types of P. globosa cell characterized by different marker pigment profiles. However, owing to a lack of quantitative methods for the detection of different types of P. globosa cells, the dynamics of P. globosa blooms in the SCS are still poorly understood. In this study, on the basis of phylogenetic analysis of the mitochondrial atp8 (Mit atp8) gene and pigment profiles, the P. globosa strains from Pacific and Atlantic coastal waters were divided into four genotypes, of which Type I and Type IV strains co-exist in the SCS. Furthermore, two genotype-specific quantitative polymerase chain reaction (qPCR) methods were developed targeting the Mit atp8 gene for quantitative determinations of the densities of Type I and Type IV P. globosa cells. Both qPCR methods were reproducible, highly sensitive, and specific with a wide range of detection (from 30 to 1 x 10(8) cells L-1) for target P. globosa genotypes in the field, thereby facilitating the detection of low cell densities prior to bloom development, as well as high cell densities during bloom periods. Finally, the developed qPCR assays were successfully applied to determine the distribution patterns of Type I and Type IV P. globosa in the Beibu Gulf, a region of the SCS characterized by a high frequency of P. globosa blooms.
DOI	10.1002/lom3.10476
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2017YFC1404304] ; National Natural Science Foundation of China[42076140] ; National Natural Science Foundation of China[U20A20104] ; National Natural Science Foundation of China[41776127] ; S&T Basic Resources Investigation Program of China[2018YF100206]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000734508400001
出版者	WILEY
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177594
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Qing-Chun
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Niu, Zhuang,Liu, Chao,Zhang, Qing-Chun,et al. Development of sensitive genotype-specific quantitative polymerase chain reaction methods for detection of Phaeocystis globosa in the South China Sea[J]. LIMNOLOGY AND OCEANOGRAPHY-METHODS,2021:15.
APA	Niu, Zhuang.,Liu, Chao.,Zhang, Qing-Chun.,Wang, Jin-Xiu.,Kong, Fan-Zhou.,...&Yu, Ren-Cheng.(2021).Development of sensitive genotype-specific quantitative polymerase chain reaction methods for detection of Phaeocystis globosa in the South China Sea.LIMNOLOGY AND OCEANOGRAPHY-METHODS,15.
MLA	Niu, Zhuang,et al."Development of sensitive genotype-specific quantitative polymerase chain reaction methods for detection of Phaeocystis globosa in the South China Sea".LIMNOLOGY AND OCEANOGRAPHY-METHODS (2021):15.

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文件名称/大小	文献类型	版本类型	开放类型	使用许可
Journal of Fish Biol（1011KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览