Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Whole-genome resequencing reveals the single nucleotide polymorphisms associated with shell shape in Crassostrea gigas | |
He, Xin1,2,3,7; Wu, Fucun1,2,5,6,7; Qi, Haigang1,2,5,6; Meng, Jie1,2,5,6 ; Wang, Wei1,2,5,6; Liu, Mingkun1,2,5,6; Li, Li1,2,4,5,6; Zhang, Guofan1,2,5,6
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| 2022-01-30 | |
| Source Publication | AQUACULTURE
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| ISSN | 0044-8486 |
| Volume | 547Pages:10 |
| Corresponding Author | Wu, Fucun(wufucun@qdio.ac.cn) |
| Abstract | Shell shape is an economically important trait in bivalves, as it can directly affect consumer behaviour. However, there have been few studies on the genes related to shell growth and shape. The Pacific oyster, Crassostrea gigas, is one of the most important aquaculture species worldwide, and studies on its shell shape could significantly increase its economic value. In this study, the shell-length-to-shell-height ratio (SLSH) was defined as the phenotype of interest. Descriptive statistics and correlation analyses of shell-related traits in the long (low phenotypic) and round (high phenotypic) groups of Crassostrea gigas are presented. Whole-genome resequencing, using individuals from these two groups, was performed to screen candidate single nucleotide polymorphisms (SNPs) and genes. A total of 925 SNPs and 593 genes associated with shell shape were found. SNaPshot was used for genotyping validation in an independent population: two SNPs were significantly related to shell shape differences, and the minor C allele of SNP g26854_18684937 favoured the growth of round shells. Further, ten genes were identified as significantly differentially expressed in the extreme shell shape groups of the independent population using real-time PCR, and the results showed that different shell shape may vary in environmental stress responses. This is the first study to use whole-genome resequencing to identify shell shape related sites in bivalves. These results not only provide a reference for genetic research on oyster shell shape but also contribute to the knowledge base for future selective breeding of bivalves. |
| Keyword | Crassostrea gigas Shell shape Whole-genome resequencing Single nucleotide polymorphism Genotyping |
| DOI | 10.1016/j.aquaculture.2021.737502 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030105] ; Major Scientific and Technological Innovation Project of Shandong Province[2019JZZY010813] ; National Key R&D Program of China[2019YFD0900800] ; Yantai Science and Technology Plan Project[2019XDHZ095] ; China Agriculture Research System of MOF ; China Agriculture Research System of MARA |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000730281100013 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/177582 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Wu, Fucun |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 4.Pilot Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 6.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 7.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | He, Xin,Wu, Fucun,Qi, Haigang,et al. Whole-genome resequencing reveals the single nucleotide polymorphisms associated with shell shape in Crassostrea gigas[J]. AQUACULTURE,2022,547:10. |
| APA | He, Xin.,Wu, Fucun.,Qi, Haigang.,Meng, Jie.,Wang, Wei.,...&Zhang, Guofan.(2022).Whole-genome resequencing reveals the single nucleotide polymorphisms associated with shell shape in Crassostrea gigas.AQUACULTURE,547,10. |
| MLA | He, Xin,et al."Whole-genome resequencing reveals the single nucleotide polymorphisms associated with shell shape in Crassostrea gigas".AQUACULTURE 547(2022):10. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 1-s2.0-S004484862101（1302KB） | 期刊论文 | 出版稿 | 延迟开放 | CC BY-NC-SA | View 2023-7-1后可获取 | |
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