Whole-genome resequencing reveals the single nucleotide polymorphisms associated with shell shape in Crassostrea gigas

doi:10.1016/j.aquaculture.2021.737502

IOCAS-IR > 实验海洋生物学重点实验室

	Whole-genome resequencing reveals the single nucleotide polymorphisms associated with shell shape in Crassostrea gigas
	He, Xin 1,2,3,7; Wu, Fucun 1,2,5,6,7; Qi, Haigang 1,2,5,6; Meng, Jie1,2,5,6 ; Wang, Wei 1,2,5,6; Liu, Mingkun 1,2,5,6; Li, Li 1,2,4,5,6; Zhang, Guofan 1,2,5,6
	2022-01-30
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	547 页码:10
通讯作者	Wu, Fucun(wufucun@qdio.ac.cn)
摘要	Shell shape is an economically important trait in bivalves, as it can directly affect consumer behaviour. However, there have been few studies on the genes related to shell growth and shape. The Pacific oyster, Crassostrea gigas, is one of the most important aquaculture species worldwide, and studies on its shell shape could significantly increase its economic value. In this study, the shell-length-to-shell-height ratio (SLSH) was defined as the phenotype of interest. Descriptive statistics and correlation analyses of shell-related traits in the long (low phenotypic) and round (high phenotypic) groups of Crassostrea gigas are presented. Whole-genome resequencing, using individuals from these two groups, was performed to screen candidate single nucleotide polymorphisms (SNPs) and genes. A total of 925 SNPs and 593 genes associated with shell shape were found. SNaPshot was used for genotyping validation in an independent population: two SNPs were significantly related to shell shape differences, and the minor C allele of SNP g26854_18684937 favoured the growth of round shells. Further, ten genes were identified as significantly differentially expressed in the extreme shell shape groups of the independent population using real-time PCR, and the results showed that different shell shape may vary in environmental stress responses. This is the first study to use whole-genome resequencing to identify shell shape related sites in bivalves. These results not only provide a reference for genetic research on oyster shell shape but also contribute to the knowledge base for future selective breeding of bivalves.
关键词	Crassostrea gigas Shell shape Whole-genome resequencing Single nucleotide polymorphism Genotyping
DOI	10.1016/j.aquaculture.2021.737502
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030105] ; Major Scientific and Technological Innovation Project of Shandong Province[2019JZZY010813] ; National Key R&D Program of China[2019YFD0900800] ; Yantai Science and Technology Plan Project[2019XDHZ095] ; China Agriculture Research System of MOF ; China Agriculture Research System of MARA
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000730281100013
出版者	ELSEVIER
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177582
专题	实验海洋生物学重点实验室
通讯作者	Wu, Fucun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 4.Pilot Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 6.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 7.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	He, Xin,Wu, Fucun,Qi, Haigang,et al. Whole-genome resequencing reveals the single nucleotide polymorphisms associated with shell shape in Crassostrea gigas[J]. AQUACULTURE,2022,547:10.
APA	He, Xin.,Wu, Fucun.,Qi, Haigang.,Meng, Jie.,Wang, Wei.,...&Zhang, Guofan.(2022).Whole-genome resequencing reveals the single nucleotide polymorphisms associated with shell shape in Crassostrea gigas.AQUACULTURE,547,10.
MLA	He, Xin,et al."Whole-genome resequencing reveals the single nucleotide polymorphisms associated with shell shape in Crassostrea gigas".AQUACULTURE 547(2022):10.

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