IOCAS-IR  > 实验海洋生物学重点实验室
Whole-genome resequencing reveals the single nucleotide polymorphisms associated with shell shape in Crassostrea gigas
He, Xin1,2,3,7; Wu, Fucun1,2,5,6,7; Qi, Haigang1,2,5,6; Meng, Jie1,2,5,6; Wang, Wei1,2,5,6; Liu, Mingkun1,2,5,6; Li, Li1,2,4,5,6; Zhang, Guofan1,2,5,6
2022-01-30
Source PublicationAQUACULTURE
ISSN0044-8486
Volume547Pages:10
Corresponding AuthorWu, Fucun(wufucun@qdio.ac.cn)
AbstractShell shape is an economically important trait in bivalves, as it can directly affect consumer behaviour. However, there have been few studies on the genes related to shell growth and shape. The Pacific oyster, Crassostrea gigas, is one of the most important aquaculture species worldwide, and studies on its shell shape could significantly increase its economic value. In this study, the shell-length-to-shell-height ratio (SLSH) was defined as the phenotype of interest. Descriptive statistics and correlation analyses of shell-related traits in the long (low phenotypic) and round (high phenotypic) groups of Crassostrea gigas are presented. Whole-genome resequencing, using individuals from these two groups, was performed to screen candidate single nucleotide polymorphisms (SNPs) and genes. A total of 925 SNPs and 593 genes associated with shell shape were found. SNaPshot was used for genotyping validation in an independent population: two SNPs were significantly related to shell shape differences, and the minor C allele of SNP g26854_18684937 favoured the growth of round shells. Further, ten genes were identified as significantly differentially expressed in the extreme shell shape groups of the independent population using real-time PCR, and the results showed that different shell shape may vary in environmental stress responses. This is the first study to use whole-genome resequencing to identify shell shape related sites in bivalves. These results not only provide a reference for genetic research on oyster shell shape but also contribute to the knowledge base for future selective breeding of bivalves.
KeywordCrassostrea gigas Shell shape Whole-genome resequencing Single nucleotide polymorphism Genotyping
DOI10.1016/j.aquaculture.2021.737502
Indexed BySCI
Language英语
Funding ProjectStrategic Priority Research Program of the Chinese Academy of Sciences[XDA24030105] ; Major Scientific and Technological Innovation Project of Shandong Province[2019JZZY010813] ; National Key R&D Program of China[2019YFD0900800] ; Yantai Science and Technology Plan Project[2019XDHZ095] ; China Agriculture Research System of MOF ; China Agriculture Research System of MARA
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000730281100013
PublisherELSEVIER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/177582
Collection实验海洋生物学重点实验室
Corresponding AuthorWu, Fucun
Affiliation1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
4.Pilot Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
6.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
7.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
He, Xin,Wu, Fucun,Qi, Haigang,et al. Whole-genome resequencing reveals the single nucleotide polymorphisms associated with shell shape in Crassostrea gigas[J]. AQUACULTURE,2022,547:10.
APA He, Xin.,Wu, Fucun.,Qi, Haigang.,Meng, Jie.,Wang, Wei.,...&Zhang, Guofan.(2022).Whole-genome resequencing reveals the single nucleotide polymorphisms associated with shell shape in Crassostrea gigas.AQUACULTURE,547,10.
MLA He, Xin,et al."Whole-genome resequencing reveals the single nucleotide polymorphisms associated with shell shape in Crassostrea gigas".AQUACULTURE 547(2022):10.
Files in This Item:
File Name/Size DocType Version Access License
1-s2.0-S004484862101(1302KB)期刊论文出版稿延迟开放CC BY-NC-SAView 2023-7-1后可获取
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[He, Xin]'s Articles
[Wu, Fucun]'s Articles
[Qi, Haigang]'s Articles
Baidu academic
Similar articles in Baidu academic
[He, Xin]'s Articles
[Wu, Fucun]'s Articles
[Qi, Haigang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[He, Xin]'s Articles
[Wu, Fucun]'s Articles
[Qi, Haigang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 1-s2.0-S0044848621011650-main.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.