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Enhancement of astaxanthin accumulation in Haematococcus pluvialis by exogenous oxaloacetate combined with nitrogen deficiency
Yu, Wenjie1,2; Zhang, Litao1,3; Zhao, Jing1; Liu, Jianguo1,3,4
2022-02-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume345Pages:10
Corresponding AuthorLiu, Jianguo(jgliu@qdio.ac.cn)
AbstractEffective inducers and stress conditions play an essential role in the regulation of astaxanthin biosynthesis. This study reports a strategy developed by combining exogenous addition of oxaloacetate (OA) with nitrogen deficiency to facilitate astaxanthin production in Haematococcus pluvialis. Significantly, addition of 10 mM-OA enhanced the cellular astaxanthin content about 7.18-fold under nitrogen deficiency on day 7, with the content of astaxanthin esters increased concomitantly. To further elucidate the role and mechanism of OA on astaxanthin synthesis, the physiological and metabolic analyses of H. pluvialis treated with exogenous OA were performed. The results showed that exogenous OA promoted respiration over photosynthesis. Concurrently, the metabolite levels in the Embden-Meyerhof-Parnas pathway, pentose phosphate pathway and tricarboxylic acid cycle obviously increased. The enhancement of respiratory metabolic pathways led to elevated levels of substrates, thus directly promoted astaxanthin synthesis. The present findings provide a new and effective approach for optimizing astaxanthin production.
KeywordOxaloacetate Astaxanthin accumulation Haematococcus pluvialis Nitrogen deficiency Respiratory metabolism
DOI10.1016/j.biortech.2021.126484
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31872588] ; National Natural Science Foundation of China[U1706209] ; TaiShan Industrial Experts Programme[tscy20200102]
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000732933200013
PublisherELSEVIER SCI LTD
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/177555
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Jianguo
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS & Shandong Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
4.Qingdao Langyatai Grp Co Ltd, Shandong Engn Technol Collaborat Innovat Ctr Edib, Qingdao 266400, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yu, Wenjie,Zhang, Litao,Zhao, Jing,et al. Enhancement of astaxanthin accumulation in Haematococcus pluvialis by exogenous oxaloacetate combined with nitrogen deficiency[J]. BIORESOURCE TECHNOLOGY,2022,345:10.
APA Yu, Wenjie,Zhang, Litao,Zhao, Jing,&Liu, Jianguo.(2022).Enhancement of astaxanthin accumulation in Haematococcus pluvialis by exogenous oxaloacetate combined with nitrogen deficiency.BIORESOURCE TECHNOLOGY,345,10.
MLA Yu, Wenjie,et al."Enhancement of astaxanthin accumulation in Haematococcus pluvialis by exogenous oxaloacetate combined with nitrogen deficiency".BIORESOURCE TECHNOLOGY 345(2022):10.
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