IOCAS-IR  > 海洋生态与环境科学重点实验室
Biodiversity exploration in autumn using environmental DNA in the South China sea
Diao, Caoyun1,2,3,4; Jia, Hui1,2,3,5; Guo, Shujin6; Hou, Gang7; Xian, Weiwei1,2,3; Zhang, Hui1,2,3,4
2022-03-01
Source PublicationENVIRONMENTAL RESEARCH
ISSN0013-9351
Volume204Pages:14
Corresponding AuthorXian, Weiwei(wwxian@qdio.ac.cn) ; Zhang, Hui(zhanghui@qdio.ac.cn)
AbstractThe South China Sea (SCS) is an important part of the Indo-Pacific convergence zone, with high biodiversity and abundant marine resources. Traditional methods are primarily used to monitor biodiversity. However, a few studies have used environmental DNA (eDNA) metabarcoding to research the assemblage structure of the SCS. This study used eDNA metabarcoding to survey the SCS assemblage and its relationship with environmental factors over a month-long time-series (August 30th to September 30th, 2020) of seawater samples from the central part of the SCS (9 degrees-20 degrees 86' N, 113 degrees-118 degrees 47' E). 32 stations were divided into six groups (A, B, C, D, E, F) according to longitude. We collected water samples, extracted eDNA, and amplified 185 rRNA gene V4 region (18S V4), 18S rRNA gene V9 region (18S V9), and 125 rRNA gene (12S). Krona diagrams were used to show species composition. We identified 192 phytoplankton, 104 invertebrate, and 61 fish species from 185 V4, 185 V9, and 125, respectively. Generally, the three assemblage structures exhibited an increase in species diversity with increasing longitude. Group E had the highest fish diversity. Groups F and C had the highest phytoplankton and invertebrate diversity, respectively. Canonical correspondence analysis showed that four factors (chlorophyll a, depth, salinity, and temperature) were correlated with assemblage structure. Chlorophyll a was the main environmental factor that affected fish, phytoplankton, and invertebrate assemblage structures; salinity was strongly correlated with fish and invertebrate assemblage structures; temperature was a key factor that impacted fish and invertebrate assemblage structures; and depth was strongly correlated with invertebrate assemblage structure. Our results revealed that eDNA metabarcoding is a powerful tool for improving detection rate and using multiple markers is an effective approach for monitoring biodiversity. This study provided information that can be used to enhance biodiversity protection efforts in the SCS.
KeywordeDNA metabarcoding Biodiversity Assemblage structure Environmental factors South China Sea
DOI10.1016/j.envres.2021.112357
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[42090044] ; National Natural Science Foundation of China[91751202] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; Youth Innovation Promotion Association CAS[2020211] ; NSFC[41949906]
WOS Research AreaEnvironmental Sciences & Ecology ; Public, Environmental & Occupational Health
WOS SubjectEnvironmental Sciences ; Public, Environmental & Occupational Health
WOS IDWOS:000730783900002
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/177546
Collection海洋生态与环境科学重点实验室
Corresponding AuthorXian, Weiwei; Zhang, Hui
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Qingdao Agr Univ, China Sch Marine Sci & Engn, Qingdao 266109, Peoples R China
6.Chinese Acad Sci, Inst Oceanol, Jiaozhou Bay Natl Marine Ecosyst Res Stn, Qingdao 266071, Peoples R China
7.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524000, Peoples R China
First Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Diao, Caoyun,Jia, Hui,Guo, Shujin,et al. Biodiversity exploration in autumn using environmental DNA in the South China sea[J]. ENVIRONMENTAL RESEARCH,2022,204:14.
APA Diao, Caoyun,Jia, Hui,Guo, Shujin,Hou, Gang,Xian, Weiwei,&Zhang, Hui.(2022).Biodiversity exploration in autumn using environmental DNA in the South China sea.ENVIRONMENTAL RESEARCH,204,14.
MLA Diao, Caoyun,et al."Biodiversity exploration in autumn using environmental DNA in the South China sea".ENVIRONMENTAL RESEARCH 204(2022):14.
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