Biodiversity exploration in autumn using environmental DNA in the South China sea

doi:10.1016/j.envres.2021.112357

	Biodiversity exploration in autumn using environmental DNA in the South China sea
	Diao, Caoyun 1,2,3,4; Jia, Hui 1,2,3,5; Guo, Shujin 6; Hou, Gang 7; Xian, Weiwei 1,2,3; Zhang, Hui1,2,3,4
	2022-03-01
发表期刊	ENVIRONMENTAL RESEARCH
ISSN	0013-9351
卷号	204 页码:14
通讯作者	Xian, Weiwei(wwxian@qdio.ac.cn) ; Zhang, Hui(zhanghui@qdio.ac.cn)
摘要	The South China Sea (SCS) is an important part of the Indo-Pacific convergence zone, with high biodiversity and abundant marine resources. Traditional methods are primarily used to monitor biodiversity. However, a few studies have used environmental DNA (eDNA) metabarcoding to research the assemblage structure of the SCS. This study used eDNA metabarcoding to survey the SCS assemblage and its relationship with environmental factors over a month-long time-series (August 30th to September 30th, 2020) of seawater samples from the central part of the SCS (9 degrees-20 degrees 86' N, 113 degrees-118 degrees 47' E). 32 stations were divided into six groups (A, B, C, D, E, F) according to longitude. We collected water samples, extracted eDNA, and amplified 185 rRNA gene V4 region (18S V4), 18S rRNA gene V9 region (18S V9), and 125 rRNA gene (12S). Krona diagrams were used to show species composition. We identified 192 phytoplankton, 104 invertebrate, and 61 fish species from 185 V4, 185 V9, and 125, respectively. Generally, the three assemblage structures exhibited an increase in species diversity with increasing longitude. Group E had the highest fish diversity. Groups F and C had the highest phytoplankton and invertebrate diversity, respectively. Canonical correspondence analysis showed that four factors (chlorophyll a, depth, salinity, and temperature) were correlated with assemblage structure. Chlorophyll a was the main environmental factor that affected fish, phytoplankton, and invertebrate assemblage structures; salinity was strongly correlated with fish and invertebrate assemblage structures; temperature was a key factor that impacted fish and invertebrate assemblage structures; and depth was strongly correlated with invertebrate assemblage structure. Our results revealed that eDNA metabarcoding is a powerful tool for improving detection rate and using multiple markers is an effective approach for monitoring biodiversity. This study provided information that can be used to enhance biodiversity protection efforts in the SCS.
关键词	eDNA metabarcoding Biodiversity Assemblage structure Environmental factors South China Sea
DOI	10.1016/j.envres.2021.112357
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[42090044] ; National Natural Science Foundation of China[91751202] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; Youth Innovation Promotion Association CAS[2020211] ; NSFC[41949906]
WOS研究方向	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
WOS类目	Environmental Sciences ; Public, Environmental & Occupational Health
WOS记录号	WOS:000730783900002
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177546
专题	海洋生态与环境科学重点实验室
通讯作者	Xian, Weiwei; Zhang, Hui
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Qingdao Agr Univ, China Sch Marine Sci & Engn, Qingdao 266109, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Jiaozhou Bay Natl Marine Ecosyst Res Stn, Qingdao 266071, Peoples R China 7.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524000, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Diao, Caoyun,Jia, Hui,Guo, Shujin,et al. Biodiversity exploration in autumn using environmental DNA in the South China sea[J]. ENVIRONMENTAL RESEARCH,2022,204:14.
APA	Diao, Caoyun,Jia, Hui,Guo, Shujin,Hou, Gang,Xian, Weiwei,&Zhang, Hui.(2022).Biodiversity exploration in autumn using environmental DNA in the South China sea.ENVIRONMENTAL RESEARCH,204,14.
MLA	Diao, Caoyun,et al."Biodiversity exploration in autumn using environmental DNA in the South China sea".ENVIRONMENTAL RESEARCH 204(2022):14.

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