Isobaric Tags for Relative and Absolute Quantitation-Based Proteomics Analysis Provides a New Perspective Into Unsynchronized Growth in Kuruma Shrimp (Marsupenaeus japonicus)

doi:10.3389/fmars.2021.761103

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	Isobaric Tags for Relative and Absolute Quantitation-Based Proteomics Analysis Provides a New Perspective Into Unsynchronized Growth in Kuruma Shrimp (Marsupenaeus japonicus)
	Zhao, Jichen 1; Liao, Minze 1; Lin, Zexu 1; Huang, Yiyi 1; Zhong, Yunqi 1; Liu, Luyao 1; Chen, Guoliang 2; Ni, Zuotao 3; Sun, Chengbo 1,4,5
	2021-12-02
发表期刊	FRONTIERS IN MARINE SCIENCE
卷号	8 页码:12
通讯作者	Ni, Zuotao(nizuotao07@qdio.ac.cn) ; Sun, Chengbo(suncb@gdou.edu.cn)
摘要	Unsynchronized growth is a common phenomenon in farmed crustaceans. The underlying molecular mechanism of unsynchronized growth of crustaceans is unclear. In this study, a comparative proteomic analysis focusing on growth differences was performed using kuruma shrimp Marsupenaeus japonicus, an economic crustacean species, as the model. The study analyzed kuruma shrimp at fast growth stage and steady growth stage from both fast growth group and slow growth group by an Isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analysis method. A total of 1,720 proteins, including 12,291 peptides, were identified. Fifty-two and 70 differentially expressed proteins (DEPs) were identified in the fast growth stage and steady growth stage, respectively. Interestingly, 10 DEPs, including 14-3-3-epsilon-like, GPI, GPD1, MHC-1a, and MHC-1b, were presented in both growth stages. In addition, all these 10 DEPs shared the same expression tendency at these two growth stages. The results indicated that these 10 DEPs are potential growth biomarkers of M. japonicus. Proteins associated with faster growth of M. japonicus may promote cell growth and inhibit cell apoptosis through the Hippo signaling pathway. The fast growth group of M. japonicus may also achieve growth superiority by activating multiple related metabolic pathways, including glycolysis, glycerophospholipid metabolism and Citrate cycle. The present study provides a new perspective to explore the molecular mechanism of unsynchronized growth in crustacean species.
关键词	Marsupenaeus japonicus unsynchronized growth proteomics growth biomarkers molecular mechanism
DOI	10.3389/fmars.2021.761103
收录类别	SCI
语种	英语
资助项目	R&D Program of Key Areas in Guangdong Province[2020B0202010009] ; R&D Program of Key Areas in Guangdong Province[2319412525]
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000730704400001
出版者	FRONTIERS MEDIA SA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177510
专题	中国科学院海洋研究所
通讯作者	Ni, Zuotao; Sun, Chengbo
作者单位	1.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang, Peoples R China 2.Guangdong Haimao Investment Co Ltd, Zhanjiang, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 4.Guangdong Prov Lab Southern Marine Sci & Engn, Zhanjiang, Peoples R China 5.Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhao, Jichen,Liao, Minze,Lin, Zexu,et al. Isobaric Tags for Relative and Absolute Quantitation-Based Proteomics Analysis Provides a New Perspective Into Unsynchronized Growth in Kuruma Shrimp (Marsupenaeus japonicus)[J]. FRONTIERS IN MARINE SCIENCE,2021,8:12.
APA	Zhao, Jichen.,Liao, Minze.,Lin, Zexu.,Huang, Yiyi.,Zhong, Yunqi.,...&Sun, Chengbo.(2021).Isobaric Tags for Relative and Absolute Quantitation-Based Proteomics Analysis Provides a New Perspective Into Unsynchronized Growth in Kuruma Shrimp (Marsupenaeus japonicus).FRONTIERS IN MARINE SCIENCE,8,12.
MLA	Zhao, Jichen,et al."Isobaric Tags for Relative and Absolute Quantitation-Based Proteomics Analysis Provides a New Perspective Into Unsynchronized Growth in Kuruma Shrimp (Marsupenaeus japonicus)".FRONTIERS IN MARINE SCIENCE 8(2021):12.

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