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Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster Crassostrea gigas
Zhang, Lingshuai1,3,4; Wang, Xiudan2; Zhang, Weiqian2; Yin, Xiaoting1,3,4; Liu, Qing1,4; Qiu, Limei1,4
2021-12-08
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Pages14
Corresponding AuthorQiu, Limei(qiulimei@qdio.ac.cn)
AbstractThe hepatopancreas is an important tissue involved in various biological metabolism for mollusks, but its responses to ocean acidification (OA) have not been well evaluated. In this study, the oysters were cultured in simulated conditions by continuously bubbling with ambient air (pH=8.10) or air-CO2 (pH=7.50) for up to two months, and the variations on the antioxidant capacity, digestive ability, and microbiota composition in hepatopancreas of Crassostrea gigas were analyzed. The results show that although superoxide dismutase and glutathione responded quickly to OA stress, the antioxidant capacity of the hepatopancreas was inhibited, as revealed by the decrease of the total antioxidant capacity, which led to an upward trend of the malondialdehyde, demonstrating that the oxidative damages were accumulated under the OA process. The determination of the digestive ability manifested as the decrease of pepsin activity and the recovery of lipase and amylase activity after long-term acidification, which may be helpful to improve the adaptability of oysters. In addition, analysis on 16S rDNA amplicon revealed that the total species abundance and diversity of the hepatopancreas microbiota experienced a dynamic change, but finally it decreased greatly after long-term acidification. The structure of the hepatopancreas microbiota was changed drastically with the change of the dominant species from aerobic to the anaerobic and facultative anaerobic bacteria, and the abnormal proliferation of some species, such as genus of Mycoplasma and order Clostridiales, which may aggravate the adverse effects of OA on the physiological functions of the hepatopancreas. As a result, our findings enrich our understanding of the accumulated oxidative damage and adaptive digestive ability in oyster hepatopancreas caused by OA. For the first time, the changes of the hepatopancreas microbiota under long-term acidification conditions are described, proving a good reference for the study of the response and adaptation mechanisms of bivalve mollusks in a wide range of oceans OA.
Keywordocean acidification oyster hepatopancreas antioxidant defense digestive enzymes microbiota
DOI10.1007/s00343-021-0462-x
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[32072999] ; National Natural Science Foundation of China[31800414] ; Shandong Province Natural Science Foundation[ZR2019BC040]
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000728779800012
PublisherSCIENCE PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/177483
Collection实验海洋生物学重点实验室
Corresponding AuthorQiu, Limei
Affiliation1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Univ Sci & Technol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Lingshuai,Wang, Xiudan,Zhang, Weiqian,et al. Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster Crassostrea gigas[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2021:14.
APA Zhang, Lingshuai,Wang, Xiudan,Zhang, Weiqian,Yin, Xiaoting,Liu, Qing,&Qiu, Limei.(2021).Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster Crassostrea gigas.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,14.
MLA Zhang, Lingshuai,et al."Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster Crassostrea gigas".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2021):14.
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