Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster Crassostrea gigas

doi:10.1007/s00343-021-0462-x

IOCAS-IR > 实验海洋生物学重点实验室

	Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster Crassostrea gigas
	Zhang, Lingshuai 1,3,4; Wang, Xiudan2 ; Zhang, Weiqian 2; Yin, Xiaoting1,3,4 ; Liu, Qing1,4 ; Qiu, Limei 1,4
	2021-12-08
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
页码	14
通讯作者	Qiu, Limei(qiulimei@qdio.ac.cn)
摘要	The hepatopancreas is an important tissue involved in various biological metabolism for mollusks, but its responses to ocean acidification (OA) have not been well evaluated. In this study, the oysters were cultured in simulated conditions by continuously bubbling with ambient air (pH=8.10) or air-CO2 (pH=7.50) for up to two months, and the variations on the antioxidant capacity, digestive ability, and microbiota composition in hepatopancreas of Crassostrea gigas were analyzed. The results show that although superoxide dismutase and glutathione responded quickly to OA stress, the antioxidant capacity of the hepatopancreas was inhibited, as revealed by the decrease of the total antioxidant capacity, which led to an upward trend of the malondialdehyde, demonstrating that the oxidative damages were accumulated under the OA process. The determination of the digestive ability manifested as the decrease of pepsin activity and the recovery of lipase and amylase activity after long-term acidification, which may be helpful to improve the adaptability of oysters. In addition, analysis on 16S rDNA amplicon revealed that the total species abundance and diversity of the hepatopancreas microbiota experienced a dynamic change, but finally it decreased greatly after long-term acidification. The structure of the hepatopancreas microbiota was changed drastically with the change of the dominant species from aerobic to the anaerobic and facultative anaerobic bacteria, and the abnormal proliferation of some species, such as genus of Mycoplasma and order Clostridiales, which may aggravate the adverse effects of OA on the physiological functions of the hepatopancreas. As a result, our findings enrich our understanding of the accumulated oxidative damage and adaptive digestive ability in oyster hepatopancreas caused by OA. For the first time, the changes of the hepatopancreas microbiota under long-term acidification conditions are described, proving a good reference for the study of the response and adaptation mechanisms of bivalve mollusks in a wide range of oceans OA.
关键词	ocean acidification oyster hepatopancreas antioxidant defense digestive enzymes microbiota
DOI	10.1007/s00343-021-0462-x
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[32072999] ; National Natural Science Foundation of China[31800414] ; Shandong Province Natural Science Foundation[ZR2019BC040]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000728779800012
出版者	SCIENCE PRESS
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177483
专题	实验海洋生物学重点实验室
通讯作者	Qiu, Limei
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Univ Sci & Technol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Lingshuai,Wang, Xiudan,Zhang, Weiqian,et al. Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster Crassostrea gigas[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2021:14.
APA	Zhang, Lingshuai,Wang, Xiudan,Zhang, Weiqian,Yin, Xiaoting,Liu, Qing,&Qiu, Limei.(2021).Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster Crassostrea gigas.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,14.
MLA	Zhang, Lingshuai,et al."Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster Crassostrea gigas".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2021):14.

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