Glutamate Dehydrogenase Functions in Glutamic Acid Metabolism and Stress Resistance in Pyropia haitanensis

doi:10.3390/molecules26226793

IOCAS-IR > 实验海洋生物学重点实验室

	Glutamate Dehydrogenase Functions in Glutamic Acid Metabolism and Stress Resistance in Pyropia haitanensis
	Li, Shuang 1,2,3; Shao, Zhanru 1,2; Lu, Chang 1,2,3; Yao, Jianting 1,2; Zhou, Yongdong 4; Duan, Delin 1,2
	2021-11-01
发表期刊	MOLECULES
卷号	26 期号:22 页码:16
通讯作者	Shao, Zhanru(zrshao@qdio.ac.cn) ; Duan, Delin(dlduan@qdio.ac.cn)
摘要	Pyropia haitanensis is an important laver species in China. Its quality traits are closely related to the content of glutamic acid. Glutamate dehydrogenase (GDH) is a crucial enzyme in the glutamic acid metabolism. In this study, two GDH genes from P. haitanensis, PhGDH1 and PhGDH2, were cloned and successfully expressed in Escherichia coli. The in vitro enzyme activity assay demonstrated that the catalytic activity of PhGDHs is mainly in the direction of ammonium assimilation. The measured K-m values of PhGDH1 for NADH, (NH4)(2)SO4, and alpha-oxoglutarate were 0.12, 4.99, and 0.16 mM, respectively, while the corresponding K-m values of PhGDH2 were 0.02, 3.98, and 0.104 mM, respectively. Site-directed mutagenesis results showed that Gly(193) and Thr(361) were important catalytic residues for PhGDH2. Moreover, expression levels of both PhGDHs were significantly increased under abiotic stresses. These results suggest that PhGDHs can convert alpha-oxoglutarate to glutamic acid, and enhance the flavor and stress resistance of P. haitanensis.
关键词	heterologous expression protein purification enzyme activity assay site-directed mutagenesis abiotic stresses
DOI	10.3390/molecules26226793
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0900106]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000725718000001
出版者	MDPI
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177294
专题	实验海洋生物学重点实验室
通讯作者	Shao, Zhanru; Duan, Delin
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Yancheng Hairui Food Co Ltd, Dafeng 224005, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Li, Shuang,Shao, Zhanru,Lu, Chang,et al. Glutamate Dehydrogenase Functions in Glutamic Acid Metabolism and Stress Resistance in Pyropia haitanensis[J]. MOLECULES,2021,26(22):16.
APA	Li, Shuang,Shao, Zhanru,Lu, Chang,Yao, Jianting,Zhou, Yongdong,&Duan, Delin.(2021).Glutamate Dehydrogenase Functions in Glutamic Acid Metabolism and Stress Resistance in Pyropia haitanensis.MOLECULES,26(22),16.
MLA	Li, Shuang,et al."Glutamate Dehydrogenase Functions in Glutamic Acid Metabolism and Stress Resistance in Pyropia haitanensis".MOLECULES 26.22(2021):16.

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