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Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome
Meng, Jie1,2,3; Zhang, Guofan3,4; Wang, Wen-Xiong1,2,5
2022
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume120Pages:202-213
Corresponding AuthorZhang, Guofan(gfzhang@qdio.ac.cn) ; Wang, Wen-Xiong(wx.wang@cityu.edu.hk)
AbstractOyster is the worldwide aquaculture molluscan and evolves a complex immune defense system, with hemocytes as the major immune system for its host defense. However, the functional heterogeneity of hemocyte has not been characterized, which markedly hinders our understanding of its defense role. Here, we used the single-cell transcriptome profiling (scRNA-seq), which provides a high-resolution visual insight into its dynamics, to map the hemocyte and assess its heterogeneity in a molluscan oyster Crassostrea hongkongensis. By combining with the cell type specific RNA-seq, thirteen subpopulations belonging to granulocyte, semi-granulocyte, and hyalinocyte were revealed. The granulocytes mainly participated in immune response and autophagy process. Pseudotemporal ordering of granulocytes identified two different cell-lineages. The hematopoietic transcription factors regulated networks controlling their differentiations were also identified. We further identified one subpopulation of granulocytes in immune activate states with the cell cycle and immune responsive genes expressions, which illustrated the functional heterogeneity of the same cell type. Collectively, our scRNA-seq analysis demonstrated the hemocytes diversity of molluscans. The results are important in our understanding of the immune defense evolution and functional differentiation of hemocytes in Phylum Mollusca.
KeywordOyster scRNA-seq Granulocytes Hematopoietic factor Pseudo-temporal ordering
DOI10.1016/j.fsi.2021.11.027
Indexed BySCI
Language英语
Funding ProjectYouth Innovation Promotion Asso-ciation of the Chinese Academy of Science[2019210]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000724951600002
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/177291
Collection实验海洋生物学重点实验室
Corresponding AuthorZhang, Guofan; Wang, Wen-Xiong
Affiliation1.City Univ Hong Kong, Sch Energy & Environm, Kowloon, Hong Kong, Peoples R China
2.City Univ Hong Kong, Hong Kong Branch Southern Marine Sci & Engn, State Key Lab Marine Pollut, Guangdong Lab Guangzhou,Kowloon, Hong Kong, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
5.City Univ Hong Kong, Res Ctr Oceans & Human Hlth, Shenzhen Res Inst, Shenzhen 518057, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Meng, Jie,Zhang, Guofan,Wang, Wen-Xiong. Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome[J]. FISH & SHELLFISH IMMUNOLOGY,2022,120:202-213.
APA Meng, Jie,Zhang, Guofan,&Wang, Wen-Xiong.(2022).Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome.FISH & SHELLFISH IMMUNOLOGY,120,202-213.
MLA Meng, Jie,et al."Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome".FISH & SHELLFISH IMMUNOLOGY 120(2022):202-213.
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