Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome | |
Meng, Jie1,2,3 ; Zhang, Guofan3,4; Wang, Wen-Xiong1,2,5
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| 2022 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| Volume | 120Pages:202-213 |
| Corresponding Author | Zhang, Guofan(gfzhang@qdio.ac.cn) ; Wang, Wen-Xiong(wx.wang@cityu.edu.hk) |
| Abstract | Oyster is the worldwide aquaculture molluscan and evolves a complex immune defense system, with hemocytes as the major immune system for its host defense. However, the functional heterogeneity of hemocyte has not been characterized, which markedly hinders our understanding of its defense role. Here, we used the single-cell transcriptome profiling (scRNA-seq), which provides a high-resolution visual insight into its dynamics, to map the hemocyte and assess its heterogeneity in a molluscan oyster Crassostrea hongkongensis. By combining with the cell type specific RNA-seq, thirteen subpopulations belonging to granulocyte, semi-granulocyte, and hyalinocyte were revealed. The granulocytes mainly participated in immune response and autophagy process. Pseudotemporal ordering of granulocytes identified two different cell-lineages. The hematopoietic transcription factors regulated networks controlling their differentiations were also identified. We further identified one subpopulation of granulocytes in immune activate states with the cell cycle and immune responsive genes expressions, which illustrated the functional heterogeneity of the same cell type. Collectively, our scRNA-seq analysis demonstrated the hemocytes diversity of molluscans. The results are important in our understanding of the immune defense evolution and functional differentiation of hemocytes in Phylum Mollusca. |
| Keyword | Oyster scRNA-seq Granulocytes Hematopoietic factor Pseudo-temporal ordering |
| DOI | 10.1016/j.fsi.2021.11.027 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Youth Innovation Promotion Asso-ciation of the Chinese Academy of Science[2019210] |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000724951600002 |
| Publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/177291 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Zhang, Guofan; Wang, Wen-Xiong |
| Affiliation | 1.City Univ Hong Kong, Sch Energy & Environm, Kowloon, Hong Kong, Peoples R China 2.City Univ Hong Kong, Hong Kong Branch Southern Marine Sci & Engn, State Key Lab Marine Pollut, Guangdong Lab Guangzhou,Kowloon, Hong Kong, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.City Univ Hong Kong, Res Ctr Oceans & Human Hlth, Shenzhen Res Inst, Shenzhen 518057, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Meng, Jie,Zhang, Guofan,Wang, Wen-Xiong. Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome[J]. FISH & SHELLFISH IMMUNOLOGY,2022,120:202-213. |
| APA | Meng, Jie,Zhang, Guofan,&Wang, Wen-Xiong.(2022).Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome.FISH & SHELLFISH IMMUNOLOGY,120,202-213. |
| MLA | Meng, Jie,et al."Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome".FISH & SHELLFISH IMMUNOLOGY 120(2022):202-213. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 1-s2.0-S105046482100（10205KB） | 期刊论文 | 出版稿 | 延迟开放 | CC BY-NC-SA | View 2023-7-1后可获取 | |
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