Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome

doi:10.1016/j.fsi.2021.11.027

IOCAS-IR > 实验海洋生物学重点实验室

	Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome
	Meng, Jie1,2,3 ; Zhang, Guofan 3,4; Wang, Wen-Xiong 1,2,5
	2022
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	120 页码:202-213
通讯作者	Zhang, Guofan(gfzhang@qdio.ac.cn) ; Wang, Wen-Xiong(wx.wang@cityu.edu.hk)
摘要	Oyster is the worldwide aquaculture molluscan and evolves a complex immune defense system, with hemocytes as the major immune system for its host defense. However, the functional heterogeneity of hemocyte has not been characterized, which markedly hinders our understanding of its defense role. Here, we used the single-cell transcriptome profiling (scRNA-seq), which provides a high-resolution visual insight into its dynamics, to map the hemocyte and assess its heterogeneity in a molluscan oyster Crassostrea hongkongensis. By combining with the cell type specific RNA-seq, thirteen subpopulations belonging to granulocyte, semi-granulocyte, and hyalinocyte were revealed. The granulocytes mainly participated in immune response and autophagy process. Pseudotemporal ordering of granulocytes identified two different cell-lineages. The hematopoietic transcription factors regulated networks controlling their differentiations were also identified. We further identified one subpopulation of granulocytes in immune activate states with the cell cycle and immune responsive genes expressions, which illustrated the functional heterogeneity of the same cell type. Collectively, our scRNA-seq analysis demonstrated the hemocytes diversity of molluscans. The results are important in our understanding of the immune defense evolution and functional differentiation of hemocytes in Phylum Mollusca.
关键词	Oyster scRNA-seq Granulocytes Hematopoietic factor Pseudo-temporal ordering
DOI	10.1016/j.fsi.2021.11.027
收录类别	SCI
语种	英语
资助项目	Youth Innovation Promotion Asso-ciation of the Chinese Academy of Science[2019210]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000724951600002
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177291
专题	实验海洋生物学重点实验室
通讯作者	Zhang, Guofan; Wang, Wen-Xiong
作者单位	1.City Univ Hong Kong, Sch Energy & Environm, Kowloon, Hong Kong, Peoples R China 2.City Univ Hong Kong, Hong Kong Branch Southern Marine Sci & Engn, State Key Lab Marine Pollut, Guangdong Lab Guangzhou,Kowloon, Hong Kong, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.City Univ Hong Kong, Res Ctr Oceans & Human Hlth, Shenzhen Res Inst, Shenzhen 518057, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Meng, Jie,Zhang, Guofan,Wang, Wen-Xiong. Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome[J]. FISH & SHELLFISH IMMUNOLOGY,2022,120:202-213.
APA	Meng, Jie,Zhang, Guofan,&Wang, Wen-Xiong.(2022).Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome.FISH & SHELLFISH IMMUNOLOGY,120,202-213.
MLA	Meng, Jie,et al."Functional heterogeneity of immune defenses in molluscan oysters Crassostrea hongkongensis revealed by high-throughput single-cell transcriptome".FISH & SHELLFISH IMMUNOLOGY 120(2022):202-213.

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