Knowledge Management System Of Institute of Oceanology, Chinese Academy of Sciences
| Combined analysis of mRNA-miRNA reveals the regulatory roles of miRNAs in the metabolism of clam Cyclina sinensis hepatopancreas during acute ammonia nitrogen stress | |
| Ge, Hongxing1,2,3,4; Shi, Junjie1; Liu, Jialing1; Liang, Xiafei1; Dong, Zhiguo1 | |
| 2021-11-26 | |
| 发表期刊 | AQUACULTURE RESEARCH
 |
| ISSN | 1355-557X |
| 页码 | 15 |
| 通讯作者 | Dong, Zhiguo(dzg7712@163.com) |
| 摘要 | Clam Cyclina sinensis behaves a stronger ammonia nitrogen tolerance, whereas the molecular regulation mechanism remains unknown. In the present study, C. sinensis was exposed to ammonia nitrogen (32.90 mg/L) for 0 h (the control), 12 h (T1) and 24 h (T2). Integrated analysis of miRNA-mRNA was conducted to reveal the regulatory roles of miRNAs in the metabolism of C. sinensis exposed to acute ammonia nitrogen. Numerous genes involved in detoxification and ammonia excretion expressed differently indicate that nitrogen assimilation and utilization is adjusted to accommodate ammonia exposure. The clam has to alter their glucose and lipid metabolism to meet energy requirements to adapt acute ammonia exposure in the environment. 12, 948 genes were identified as target genes of the differently expressed miRNAs. Most of mRNA-miRNA pairs were involved in metabolisms of carbohydrate, lipid and amino acid. miRNA-mRNA integrated analysis revealed involvement of Purine metabolism, Wnt signalling pathway, Hippo signalling pathway, PI3K-Akt signalling pathway in ammonia-stress response of C. sinensis. Exposed to ammonia, clam may suffer hypoxia exposure. Overall, exposed to ammonia, clam has to alter their glucose and lipid metabolism to meet energy requirements. Numerous mRNA-miRNA pairs were involved in metabolisms of carbohydrate, lipid and amino acid and miRNA can regulate the metabolism of C. sinensis by regulating the expression of target genes during acute ammonia nitrogen stress. The net result is that nitrogen assimilation and utilization is adjusted to accommodate ammonia exposure. The current study is helpful for further investigation into the ammonia nitrogen response mechanisms in mollusks. |
| 关键词 | clam metabolism miRNA molecular response mRNA NH3 |
| DOI | 10.1111/are.15683 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Key Research and Development projects[2019YFD0900403] ; China Agriculture Research System of MOF ; China Agriculture Research System of MARA ; Priority and Academic Program Development of Jiangsu Higher Education Institutions[20KJB240002] ; Opening Foundation of Jiangsu Institute of Marine Resources Development[JSIMR202017] |
| WOS研究方向 | Fisheries |
| WOS类目 | Fisheries |
| WOS记录号 | WOS:000722748800001 |
| 出版者 | WILEY |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/177273 |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Dong, Zhiguo |
| 作者单位 | 1.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Bioresources & Ecoenvironm, Lianyungang 222005, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 3.Jiangsu Inst Marine Resources Dev, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang, Peoples R China 4.Jiangsu Inst Marine Resources Dev, Lianyungang, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Ge, Hongxing,Shi, Junjie,Liu, Jialing,et al. Combined analysis of mRNA-miRNA reveals the regulatory roles of miRNAs in the metabolism of clam Cyclina sinensis hepatopancreas during acute ammonia nitrogen stress[J]. AQUACULTURE RESEARCH,2021:15. |
| APA | Ge, Hongxing,Shi, Junjie,Liu, Jialing,Liang, Xiafei,&Dong, Zhiguo.(2021).Combined analysis of mRNA-miRNA reveals the regulatory roles of miRNAs in the metabolism of clam Cyclina sinensis hepatopancreas during acute ammonia nitrogen stress.AQUACULTURE RESEARCH,15. |
| MLA | Ge, Hongxing,et al."Combined analysis of mRNA-miRNA reveals the regulatory roles of miRNAs in the metabolism of clam Cyclina sinensis hepatopancreas during acute ammonia nitrogen stress".AQUACULTURE RESEARCH (2021):15. |
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| Aquaculture Research（1064KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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