Loading Effect of Chitosan Derivative Nanoparticles on Different Antigens and Their Immunomodulatory Activity on Dendritic Cells

doi:10.3390/md19100536

IOCAS-IR > 实验海洋生物学重点实验室

	Loading Effect of Chitosan Derivative Nanoparticles on Different Antigens and Their Immunomodulatory Activity on Dendritic Cells
	Xu, Chaojie 1,2,3; Xing, Ronge1,2 ; Liu, Song 1,2; Qin, Yukun1,2 ; Li, Kecheng 1,2; Yu, Huahua1,2 ; Li, Pengcheng1,2
	2021-10-01
发表期刊	MARINE DRUGS
卷号	19 期号:10 页码:15
通讯作者	Xing, Ronge(xingronge@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
摘要	Drug carrier nanoparticles (NPs) were prepared by the polyelectrolyte method, with chitosan sulfate, with different substituents and quaternary ammonium chitosan, including C236-HACC NPs, C36-HACC NPs, and C6-HACC NPs. To evaluate whether the NPs are suitable for loading different antigens, we chose bovine serum albumin (BSA), ovalbumin (OVA), and myoglobin (Mb) as model antigens to investigate the encapsulation effect of the NPs. The characteristics (size, potential, and encapsulation efficiency) of the NPs were measured. Moreover, the NPs with higher encapsulation efficiency were selected for the immunological activity research. The results showed that chitosan derivative NPs with different substitution sites had different loading effects on the three antigens, and the encapsulation rate of BSA and OVA was significantly better than that of Mb. Moreover, the NPs encapsulated with different antigens have different immune stimulating abilities to DCS cells, the immune effect of OVA-coated NPs was significantly better than that of BSA-coated NPs and blank NPs, especially C236-HACC-OVA NPs. Furthermore, we found that C236-HACC-OVA NPs could increase the phosphorylation level of intracellular proteins to activate cell pathways. Therefore, C236-HACC NPs are more suitable for the loading of antigens similar to the OVA structure.
关键词	chitosan derivative nanoparticles different antigens antigen selectivity encapsulation efficiency immune effects
DOI	10.3390/md19100536
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFC0311300] ; Key deployment projects of Marine Science Research Center of Chinese Academy of Sciences[COMS2020J04]
WOS研究方向	Pharmacology & Pharmacy
WOS类目	Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS记录号	WOS:000717005200001
出版者	MDPI
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177261
专题	实验海洋生物学重点实验室
通讯作者	Xing, Ronge; Li, Pengcheng
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Xu, Chaojie,Xing, Ronge,Liu, Song,et al. Loading Effect of Chitosan Derivative Nanoparticles on Different Antigens and Their Immunomodulatory Activity on Dendritic Cells[J]. MARINE DRUGS,2021,19(10):15.
APA	Xu, Chaojie.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2021).Loading Effect of Chitosan Derivative Nanoparticles on Different Antigens and Their Immunomodulatory Activity on Dendritic Cells.MARINE DRUGS,19(10),15.
MLA	Xu, Chaojie,et al."Loading Effect of Chitosan Derivative Nanoparticles on Different Antigens and Their Immunomodulatory Activity on Dendritic Cells".MARINE DRUGS 19.10(2021):15.

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