Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I | |
Liu, Ge1,2,3,4,5; Liu, Rui1,2,3,4,5; Shan, Yeqi1,2,3,4,5,6; Sun, Chaomin1,2,3,4,5,6 | |
2021-10-01 | |
发表期刊 | JOURNAL OF BIOLOGICAL CHEMISTRY |
卷号 | 297期号:4页码:12 |
通讯作者 | Sun, Chaomin(sunchaomin@qdio.ac.cn) |
摘要 | Many natural polysaccharides have significant anticancer activity with low toxicity, but the complex chemical structures make in-depth studies of the involved mechanisms extremely difficult. The purpose of this study was to investigate the effect of the marine bacterial exopolysaccharide (exopolysaccharide 11 [EPS11]) on liver cancer metastasis to explore the underly-ing target protein and molecular mechanism. We found that EPS11 significantly suppressed cell adhesion, migration, and invasion in liver cancer cells. Proteomic analysis showed that EPS11 induced downregulation of proteins related to the extracellular matrix-receptor interaction signaling pathway. In addition, the direct pharmacological target of EPS11 was identified as collagen I using cellular thermal shift assays. Surface plasmon resonance and pull-down assays further confirmed the specific binding of EPS11 to collagen I. More-over, EPS11 was shown to inhibit tumor metastasis by directly modulating collagen I activity via the beta 1-integrin-mediated signaling pathway. Collectively, our study demonstrated for the first time that collagen I could be a direct pharmacological target of polysaccharide drugs. Moreover, directly targeting collagen I may be a promising strategy for finding novel carbohydrate-based drugs. |
DOI | 10.1016/j.jbc.2021.101133 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[42006081] ; China Ocean Mineral Resources RD Association[DY135-B2-14] ; National Key R and D Program of China[2018YFC0310800] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051] ; Natural Science Foundation of Shandong Province[ZR2019BH024] ; Basic Applied Research program of Qingdao[19-6-2-35-cg] |
WOS研究方向 | Biochemistry & Molecular Biology |
WOS类目 | Biochemistry & Molecular Biology |
WOS记录号 | WOS:000713100000012 |
出版者 | ELSEVIER |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/177195 |
专题 | 实验海洋生物学重点实验室 深海极端环境与生命过程研究中心 |
通讯作者 | Sun, Chaomin |
作者单位 | 1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 6.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China |
第一作者单位 | 中国科学院海洋研究所; 深海极端环境与生命过程研究中心; 中国科学院海洋大科学研究中心 |
通讯作者单位 | 中国科学院海洋研究所; 深海极端环境与生命过程研究中心; 中国科学院海洋大科学研究中心 |
推荐引用方式 GB/T 7714 | Liu, Ge,Liu, Rui,Shan, Yeqi,et al. Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2021,297(4):12. |
APA | Liu, Ge,Liu, Rui,Shan, Yeqi,&Sun, Chaomin.(2021).Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I.JOURNAL OF BIOLOGICAL CHEMISTRY,297(4),12. |
MLA | Liu, Ge,et al."Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I".JOURNAL OF BIOLOGICAL CHEMISTRY 297.4(2021):12. |
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