IOCAS-IR  > 实验海洋生物学重点实验室
Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I
Liu, Ge1,2,3,4,5; Liu, Rui1,2,3,4,5; Shan, Yeqi1,2,3,4,5,6; Sun, Chaomin1,2,3,4,5,6
2021-10-01
发表期刊JOURNAL OF BIOLOGICAL CHEMISTRY
卷号297期号:4页码:12
通讯作者Sun, Chaomin(sunchaomin@qdio.ac.cn)
摘要Many natural polysaccharides have significant anticancer activity with low toxicity, but the complex chemical structures make in-depth studies of the involved mechanisms extremely difficult. The purpose of this study was to investigate the effect of the marine bacterial exopolysaccharide (exopolysaccharide 11 [EPS11]) on liver cancer metastasis to explore the underly-ing target protein and molecular mechanism. We found that EPS11 significantly suppressed cell adhesion, migration, and invasion in liver cancer cells. Proteomic analysis showed that EPS11 induced downregulation of proteins related to the extracellular matrix-receptor interaction signaling pathway. In addition, the direct pharmacological target of EPS11 was identified as collagen I using cellular thermal shift assays. Surface plasmon resonance and pull-down assays further confirmed the specific binding of EPS11 to collagen I. More-over, EPS11 was shown to inhibit tumor metastasis by directly modulating collagen I activity via the beta 1-integrin-mediated signaling pathway. Collectively, our study demonstrated for the first time that collagen I could be a direct pharmacological target of polysaccharide drugs. Moreover, directly targeting collagen I may be a promising strategy for finding novel carbohydrate-based drugs.
DOI10.1016/j.jbc.2021.101133
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[42006081] ; China Ocean Mineral Resources RD Association[DY135-B2-14] ; National Key R and D Program of China[2018YFC0310800] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051] ; Natural Science Foundation of Shandong Province[ZR2019BH024] ; Basic Applied Research program of Qingdao[19-6-2-35-cg]
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemistry & Molecular Biology
WOS记录号WOS:000713100000012
出版者ELSEVIER
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/177195
专题实验海洋生物学重点实验室
深海极端环境与生命过程研究中心
通讯作者Sun, Chaomin
作者单位1.Chinese Acad Sci, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
6.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
第一作者单位中国科学院海洋研究所;  深海极端环境与生命过程研究中心;  中国科学院海洋大科学研究中心
通讯作者单位中国科学院海洋研究所;  深海极端环境与生命过程研究中心;  中国科学院海洋大科学研究中心
推荐引用方式
GB/T 7714
Liu, Ge,Liu, Rui,Shan, Yeqi,et al. Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2021,297(4):12.
APA Liu, Ge,Liu, Rui,Shan, Yeqi,&Sun, Chaomin.(2021).Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I.JOURNAL OF BIOLOGICAL CHEMISTRY,297(4),12.
MLA Liu, Ge,et al."Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I".JOURNAL OF BIOLOGICAL CHEMISTRY 297.4(2021):12.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
1-s2.0-S002192582100(3926KB)期刊论文出版稿限制开放CC BY-NC-SA浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Ge]的文章
[Liu, Rui]的文章
[Shan, Yeqi]的文章
百度学术
百度学术中相似的文章
[Liu, Ge]的文章
[Liu, Rui]的文章
[Shan, Yeqi]的文章
必应学术
必应学术中相似的文章
[Liu, Ge]的文章
[Liu, Rui]的文章
[Shan, Yeqi]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 1-s2.0-S0021925821009340-main.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。