Expression Patterns of the Heat Shock Protein 90 (Hsp90) Gene Suggest Its Possible Involvement in Maintaining the Dormancy of Dinoflagellate Resting Cysts

doi:10.3390/ijms222011054

	Expression Patterns of the Heat Shock Protein 90 (Hsp90) Gene Suggest Its Possible Involvement in Maintaining the Dormancy of Dinoflagellate Resting Cysts
	Deng, Yunyan 1,2,3; Li, Fengting 1,4; Hu, Zhangxi1,2,3 ; Yue, Caixia 1,4; Tang, Ying Zhong 1,2,3
	2021-10-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	22 期号:20 页码:18
通讯作者	Tang, Ying Zhong(yingzhong.tang@qdio.ac.cn)
摘要	Heat shock protein 90 (Hsp90) is a highly conserved molecular chaperone functioning in cellular structural folding and conformational integrity maintenance and thus plays vital roles in a variety of biological processes. However, many aspects of these functions and processes remain to be fully elucidated, particularly for non-model organisms. Dinoflagellates are a group of eukaryotes that are exceedingly important in primary production and are responsible for the most harmful algal blooms (HABs) in aquatic ecosystems. The success of dinoflagellates in dominating the plankton community is undoubtedly pertinent to their remarkable adaptive strategies, characteristic of resting cyst production and broad tolerance to stresses of temperature and others. Therefore, this study was conducted to examine the putative roles of Hsp90 in the acclimation to temperature stress and life stage alterations of dinoflagellates. Firstly, we isolated the full-length cDNA of an Hsp90 gene (StHsp90) via RACE from the cosmopolitan HAB species Scrippsiella trochoidea and tracked its transcriptions in response to varied scenarios via real-time qPCR. The results indicated that StHsp90 displayed significant mRNA augment patterns, escalating during 180-min treatments, when the cells were exposed to elevated and lowered temperatures. Secondly, we observed prominently elevated StHsp90 transcriptions in the cysts that were stored at the cold and dark conditions compared to those in newly formed resting cysts and vegetative cells. Finally, and perhaps most importantly, we identified 29 entries of Hsp90-encoding genes with complete coding regions from a dinoflagellate-specific environmental cDNA library generated from marine sediment assemblages. The observed active transcription of these genes in sediment-buried resting cysts was fully supported by the qPCR results for the cold-stored resting cysts of S. trochoidea. Hsp90s expressions in both laboratory-raised and field-collected cysts collectively highlighted the possible involvement and engagement of Hsp90 chaperones in the resting stage persistence of dinoflagellates.
关键词	dinoflagellate dormancy environmental cDNA library heat shock protein 90 (Hsp90) resting cysts Scrippsiella trochoidea temperature stress
DOI	10.3390/ijms222011054
收录类别	SCI
语种	英语
资助项目	Key Deployment Project of the Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2019Q09] ; National Science Foundation of China[42176207]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000715988100001
出版者	MDPI
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177168
专题	海洋生态与环境科学重点实验室
通讯作者	Tang, Ying Zhong
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Deng, Yunyan,Li, Fengting,Hu, Zhangxi,et al. Expression Patterns of the Heat Shock Protein 90 (Hsp90) Gene Suggest Its Possible Involvement in Maintaining the Dormancy of Dinoflagellate Resting Cysts[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(20):18.
APA	Deng, Yunyan,Li, Fengting,Hu, Zhangxi,Yue, Caixia,&Tang, Ying Zhong.(2021).Expression Patterns of the Heat Shock Protein 90 (Hsp90) Gene Suggest Its Possible Involvement in Maintaining the Dormancy of Dinoflagellate Resting Cysts.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(20),18.
MLA	Deng, Yunyan,et al."Expression Patterns of the Heat Shock Protein 90 (Hsp90) Gene Suggest Its Possible Involvement in Maintaining the Dormancy of Dinoflagellate Resting Cysts".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.20(2021):18.

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