Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
A Neoglycoprotein-Immobilized Fluorescent Magnetic Bead Suspension Multiplex Array for Galectin-Binding Studies | |
Zhang, Libo1; Yu, Hai1; Bai, Yuanyuan1; Mishra, Bijoyananda1; Yang, Xiaoxiao1; Wang, Jing1,2; Yu, Evan B.1; Li, Riyao1; Chen, Xi1 | |
2021-10-01 | |
发表期刊 | MOLECULES |
卷号 | 26期号:20页码:20 |
通讯作者 | Chen, Xi(xiichen@ucdavis.edu) |
摘要 | Carbohydrate-protein conjugates have diverse applications. They have been used clinically as vaccines against bacterial infection and have been developed for high-throughput assays to elucidate the ligand specificities of glycan-binding proteins (GBPs) and antibodies. Here, we report an effective process that combines highly efficient chemoenzymatic synthesis of carbohydrates, production of carbohydrate-bovine serum albumin (glycan-BSA) conjugates using a squarate linker, and convenient immobilization of the resulting neoglycoproteins on carboxylate-coated fluorescent magnetic beads for the development of a suspension multiplex array platform. A glycan-BSA-bead array containing BSA and 50 glycan-BSA conjugates with tuned glycan valency was generated. The binding profiles of six plant lectins with binding preference towards Gal and/or GalNAc, as well as human galectin-3 and galectin-8, were readily obtained. Our results provide useful information to understand the multivalent glycan-binding properties of human galectins. The neoglycoprotein-immobilized fluorescent magnetic bead suspension multiplex array is a robust and flexible platform for rapid analysis of glycan and GBP interactions and will find broad applications. |
关键词 | carbohydrate-protein conjugate galectin glycan-binding protein lectin multiplex assay |
DOI | 10.3390/molecules26206194 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | United States National Institutes of health (NIH)[R01AI130684] ; United States NIH Shared Instrumentation Grant[S10OD025271] ; United States NIH Shared Instrumentation Grant[S10OD018913] ; United States National Science Foundation[DBI-0722538] |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
WOS记录号 | WOS:000713991300001 |
出版者 | MDPI |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/177144 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Chen, Xi |
作者单位 | 1.Univ Calif Davis, Dept Chem, Davis, CA 95616 USA 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Libo,Yu, Hai,Bai, Yuanyuan,et al. A Neoglycoprotein-Immobilized Fluorescent Magnetic Bead Suspension Multiplex Array for Galectin-Binding Studies[J]. MOLECULES,2021,26(20):20. |
APA | Zhang, Libo.,Yu, Hai.,Bai, Yuanyuan.,Mishra, Bijoyananda.,Yang, Xiaoxiao.,...&Chen, Xi.(2021).A Neoglycoprotein-Immobilized Fluorescent Magnetic Bead Suspension Multiplex Array for Galectin-Binding Studies.MOLECULES,26(20),20. |
MLA | Zhang, Libo,et al."A Neoglycoprotein-Immobilized Fluorescent Magnetic Bead Suspension Multiplex Array for Galectin-Binding Studies".MOLECULES 26.20(2021):20. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
molecules-26-06194-v(1361KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 |
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