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A Neoglycoprotein-Immobilized Fluorescent Magnetic Bead Suspension Multiplex Array for Galectin-Binding Studies
Zhang, Libo1; Yu, Hai1; Bai, Yuanyuan1; Mishra, Bijoyananda1; Yang, Xiaoxiao1; Wang, Jing1,2; Yu, Evan B.1; Li, Riyao1; Chen, Xi1
2021-10-01
Source PublicationMOLECULES
Volume26Issue:20Pages:20
Corresponding AuthorChen, Xi(xiichen@ucdavis.edu)
AbstractCarbohydrate-protein conjugates have diverse applications. They have been used clinically as vaccines against bacterial infection and have been developed for high-throughput assays to elucidate the ligand specificities of glycan-binding proteins (GBPs) and antibodies. Here, we report an effective process that combines highly efficient chemoenzymatic synthesis of carbohydrates, production of carbohydrate-bovine serum albumin (glycan-BSA) conjugates using a squarate linker, and convenient immobilization of the resulting neoglycoproteins on carboxylate-coated fluorescent magnetic beads for the development of a suspension multiplex array platform. A glycan-BSA-bead array containing BSA and 50 glycan-BSA conjugates with tuned glycan valency was generated. The binding profiles of six plant lectins with binding preference towards Gal and/or GalNAc, as well as human galectin-3 and galectin-8, were readily obtained. Our results provide useful information to understand the multivalent glycan-binding properties of human galectins. The neoglycoprotein-immobilized fluorescent magnetic bead suspension multiplex array is a robust and flexible platform for rapid analysis of glycan and GBP interactions and will find broad applications.

Keywordcarbohydrate-protein conjugate galectin glycan-binding protein lectin multiplex assay
DOI10.3390/molecules26206194
Indexed BySCI
Language英语
Funding ProjectUnited States National Institutes of health (NIH)[R01AI130684] ; United States NIH Shared Instrumentation Grant[S10OD025271] ; United States NIH Shared Instrumentation Grant[S10OD018913] ; United States National Science Foundation[DBI-0722538]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000713991300001
PublisherMDPI
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/177144
Collection实验海洋生物学重点实验室
Corresponding AuthorChen, Xi
Affiliation1.Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Libo,Yu, Hai,Bai, Yuanyuan,et al. A Neoglycoprotein-Immobilized Fluorescent Magnetic Bead Suspension Multiplex Array for Galectin-Binding Studies[J]. MOLECULES,2021,26(20):20.
APA Zhang, Libo.,Yu, Hai.,Bai, Yuanyuan.,Mishra, Bijoyananda.,Yang, Xiaoxiao.,...&Chen, Xi.(2021).A Neoglycoprotein-Immobilized Fluorescent Magnetic Bead Suspension Multiplex Array for Galectin-Binding Studies.MOLECULES,26(20),20.
MLA Zhang, Libo,et al."A Neoglycoprotein-Immobilized Fluorescent Magnetic Bead Suspension Multiplex Array for Galectin-Binding Studies".MOLECULES 26.20(2021):20.
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