The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas

doi:10.1016/j.dci.2021.104145

IOCAS-IR > 实验海洋生物学重点实验室

	The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas
	Xin, Lusheng1,2 ; Liu, Chang 3,4,5; Zhang, Huan 1; Qiu, Limei 1; Wang, Lingling 2,3,4,5; Song, Linsheng 2,3,4,5
	2021-10-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	123 页码:8
通讯作者	Song, Linsheng(lshsong@dlou.edu.cn)
摘要	Vertebrate interleukin-12 (IL-12) is a heterodimeric cytokine composing of two subunits (p35 and p40). In the present study, a p35-like subunit homolog of vertebrate IL-12 was identified from oyster Crassostrea gigas (designated as CgIL12p35L), with an open reading frame of 411 bp encoding a putative peptide of 136 amino acids. There was a long four-helix chain in CgIL12p35L, which was similar as that in vertebrate IL-12 p35. Comparative genomic analysis showed that there were conservative kinds of syntenic genes flanked CgIL12p35L. The mRNA transcripts of CgIL12p35L were constitutively expressed in various tissues and its mRNA expression level in haemocytes increased significantly after bacteria challenge. The activity of haemolymph to eliminate bacteria from the oysters treated with recombinant CgIL12p35L protein (rCgIL12p35L) in vivo increased significantly. The results collectively indicated that the homolog of vertebrate IL-12 p35 subunit existed in oysters, and it was involved in immune defense against bacteria challenge.
关键词	Crassostrea gigas Cytokine IL-12 IL-12 p35 subunit
DOI	10.1016/j.dci.2021.104145
收录类别	SCI
语种	英语
资助项目	National Science Foundation of China[U1706204] ; National Key RD Program[2018YFD0900504] ; China Agriculture Research System of MOF and MARA ; Outstanding Talents and Innovative teams of Agricultural Scientific Research in Ministry of Agriculture ; Research Foundation for Distinguished Professor in Liaoning[XLYC1902012] ; Research Foundation for Climbing Scholars in Liaoning and Talented Scholars in Dalian Ocean University
WOS研究方向	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS类目	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS记录号	WOS:000672560600004
出版者	ELSEVIER SCI LTD
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177027
专题	实验海洋生物学重点实验室
通讯作者	Song, Linsheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China 3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China 4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China 5.Dalian Ocean Univ, Dalian Key Lab Aquat Anim Dis Prevent & Control, Dalian 116023, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Xin, Lusheng,Liu, Chang,Zhang, Huan,et al. The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,123:8.
APA	Xin, Lusheng,Liu, Chang,Zhang, Huan,Qiu, Limei,Wang, Lingling,&Song, Linsheng.(2021).The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,123,8.
MLA	Xin, Lusheng,et al."The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 123(2021):8.

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