Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas | |
Xin, Lusheng1,2 ; Liu, Chang3,4,5; Zhang, Huan1; Qiu, Limei1; Wang, Lingling2,3,4,5; Song, Linsheng2,3,4,5
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| 2021-10-01 | |
| Source Publication | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| ISSN | 0145-305X |
| Volume | 123Pages:8 |
| Corresponding Author | Song, Linsheng(lshsong@dlou.edu.cn) |
| Abstract | Vertebrate interleukin-12 (IL-12) is a heterodimeric cytokine composing of two subunits (p35 and p40). In the present study, a p35-like subunit homolog of vertebrate IL-12 was identified from oyster Crassostrea gigas (designated as CgIL12p35L), with an open reading frame of 411 bp encoding a putative peptide of 136 amino acids. There was a long four-helix chain in CgIL12p35L, which was similar as that in vertebrate IL-12 p35. Comparative genomic analysis showed that there were conservative kinds of syntenic genes flanked CgIL12p35L. The mRNA transcripts of CgIL12p35L were constitutively expressed in various tissues and its mRNA expression level in haemocytes increased significantly after bacteria challenge. The activity of haemolymph to eliminate bacteria from the oysters treated with recombinant CgIL12p35L protein (rCgIL12p35L) in vivo increased significantly. The results collectively indicated that the homolog of vertebrate IL-12 p35 subunit existed in oysters, and it was involved in immune defense against bacteria challenge. |
| Keyword | Crassostrea gigas Cytokine IL-12 IL-12 p35 subunit |
| DOI | 10.1016/j.dci.2021.104145 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Science Foundation of China[U1706204] ; National Key RD Program[2018YFD0900504] ; China Agriculture Research System of MOF and MARA ; Outstanding Talents and Innovative teams of Agricultural Scientific Research in Ministry of Agriculture ; Research Foundation for Distinguished Professor in Liaoning[XLYC1902012] ; Research Foundation for Climbing Scholars in Liaoning and Talented Scholars in Dalian Ocean University |
| WOS Research Area | Fisheries ; Immunology ; Veterinary Sciences ; Zoology |
| WOS Subject | Fisheries ; Immunology ; Veterinary Sciences ; Zoology |
| WOS ID | WOS:000672560600004 |
| Publisher | ELSEVIER SCI LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/177027 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Song, Linsheng |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China 3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China 4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China 5.Dalian Ocean Univ, Dalian Key Lab Aquat Anim Dis Prevent & Control, Dalian 116023, Peoples R China |
| First Author Affilication | Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Xin, Lusheng,Liu, Chang,Zhang, Huan,et al. The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,123:8. |
| APA | Xin, Lusheng,Liu, Chang,Zhang, Huan,Qiu, Limei,Wang, Lingling,&Song, Linsheng.(2021).The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,123,8. |
| MLA | Xin, Lusheng,et al."The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 123(2021):8. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 1-s2.0-S0145305X2100（7721KB） | 期刊论文 | 出版稿 | 延迟开放 | CC BY-NC-SA | View 2023-7-1后可获取 | |
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