Identification and characterization of immune-related lncRNAs and lncRNA-miRNA-mRNA networks of Paralichthys olivaceus involved in Vibrio anguillarum infection

doi:10.1186/s12864-021-07780-2

IOCAS-IR > 实验海洋生物学重点实验室

	Identification and characterization of immune-related lncRNAs and lncRNA-miRNA-mRNA networks of Paralichthys olivaceus involved in Vibrio anguillarum infection
	Ning, Xianhui 1,2,3,4; Sun, Li 1,3
	2021-06-15
发表期刊	BMC GENOMICS
ISSN	1471-2164
卷号	22 期号:1 页码:13
通讯作者	Sun, Li(lsun@qdio.ac.cn)
摘要	Background Long non-coding RNAs (lncRNAs) structurally resemble mRNAs and exert crucial effects on host immune defense against pathogen infection. Japanese flounder (Paralichthys olivaceus) is an economically important marine fish susceptible to Vibrio anguillarum infection. To date, study on lncRNAs in flounder is scarce. Results Here, we reported the first systematic identification and characterization of flounder lncRNAs induced by V. anguillarum infection at different time points. A total of 2,368 lncRNAs were identified, 414 of which were differentially expressed lncRNAs (DElncRNAs) that responded significantly to V. anguillarum infection. For these DElncRNAs, 3,990 target genes (named DETGs) and 42 target miRNAs (named DETmiRs) were identified based on integrated analyses of lncRNA-mRNA and lncRNA-miRNA expressions, respectively. The DETGs were enriched in a cohort of functional pathways associated with immunity. In addition to modulating mRNAs, 36 DElncRNAs were also found to act as competitive endogenous RNAs (ceRNAs) that regulate 37 DETGs through 16 DETmiRs. The DETmiRs, DElncRNAs, and DETGs formed ceRNA regulatory networks consisting of 114 interacting DElncRNAs-DETmiRs-DETGs trinities spanning 10 immune pathways. Conclusions This study provides a comprehensive picture of lncRNAs involved in V. anguillarum infection. The identified lncRNAs and ceRNA networks add new insights into the anti-bacterial immunity of flounder.
关键词	LncRNA Paralichthys olivaceus Vibrio anguillarum Immune pathway ceRNA network
DOI	10.1186/s12864-021-07780-2
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31730100] ; National Natural Science Foundation of China[42006082] ; China Postdoctoral Science Foundation[2019M662456] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:000664816400001
出版者	BMC
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177012
专题	实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Nanjing Normal Univ, Coll Marine Sci & Engn, Nanjing 210023, Jiangsu, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Coinnovat Ctr Marine Bioind Technol Jiangsu Prov, Lianyungang 222005, Jiangsu, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Ning, Xianhui,Sun, Li. Identification and characterization of immune-related lncRNAs and lncRNA-miRNA-mRNA networks of Paralichthys olivaceus involved in Vibrio anguillarum infection[J]. BMC GENOMICS,2021,22(1):13.
APA	Ning, Xianhui,&Sun, Li.(2021).Identification and characterization of immune-related lncRNAs and lncRNA-miRNA-mRNA networks of Paralichthys olivaceus involved in Vibrio anguillarum infection.BMC GENOMICS,22(1),13.
MLA	Ning, Xianhui,et al."Identification and characterization of immune-related lncRNAs and lncRNA-miRNA-mRNA networks of Paralichthys olivaceus involved in Vibrio anguillarum infection".BMC GENOMICS 22.1(2021):13.

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