Threonine dehydratase enhances bacterial cadmium resistance via driving cysteine desulfuration and biomineralization of cadmium sulfide nanocrystals

doi:10.1016/j.jhazmat.2021.126102

IOCAS-IR > 实验海洋生物学重点实验室

	Threonine dehydratase enhances bacterial cadmium resistance via driving cysteine desulfuration and biomineralization of cadmium sulfide nanocrystals
	Ma, Ning 1,2,3,4,5; Cai, Ruining 1,2,3,4,5; Sun, Chaomin 1,2,3,5
	2021-09-05
发表期刊	JOURNAL OF HAZARDOUS MATERIALS
ISSN	0304-3894
卷号	417 页码:10
通讯作者	Sun, Chaomin(sunchaomin@qdio.ac.cn)
摘要	Biomineralization is often used by microorganisms to sequester heavy metal ions and provides a potential means for remediating increasing levels of heavy metal pollution. Bacteria have been shown to utilize cysteine for the biomineralization of metal sulfide. Indeed, in the present study, the supplement of L-cysteine was found to significantly improve both cadmium resistance and removal abilities of a deep-sea bacterium Pseudomonas stutzeri 273 through cadmium sulfide (CdS) nanoparticle biomineralization. With a proteomic approach, threonine dehydratase of P. stutzeri 273 (psTD) was proposed to be a key factor enhancing bacterial cadmium resistance through catalyzing L-cysteine desulfuration, H2S generation and CdS nanoparticle biomineralization. Consistently, deletion of the gene encoding psTD in P. stutzeri 273 resulted in the decline of H2S generation, decrease of cadmium resistance, and reduction of cadmium removal ability, confirming the unique function of psTD directing the formation of CdS nanoparticles. Correspondingly, the single-enzyme biomineralization of CdS nanoparticle driven by psTD was further developed, and psTD was shown to act as a capping reagent for the mineralization reaction, which controlling the size and structure of nanocrystals. Our results provide important clues for the construction of engineered bacteria for cadmium bioremediation and widen the synthesis methods of nanomaterials.
关键词	Threonine dehydratase Cadmium resistance Hydrogen sulfide Cadmium sulfide nanocrystal Biomineralization
DOI	10.1016/j.jhazmat.2021.126102
收录类别	SCI
语种	英语
资助项目	China Ocean Mineral Resources R&D Association Grant[DY135-B2-14] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; Major Research Plan of the National Natural Science Foundation of China[92051107] ; Key Deployment Projects of Center of Ocean Mega-Science of the Chinese Academy of Sciences[COMS2020Q04] ; National Key Research and Development Program of China[2018YFC0310800] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051] ; Qingdao Innovation Leadership Program[18-1-2-7-zhc] ; Open Research Project of National Major Science & Technology Infrastructure (RV KEXUE)[NMSTI-KEXUE2017K01]
WOS研究方向	Engineering ; Environmental Sciences & Ecology
WOS类目	Engineering, Environmental ; Environmental Sciences
WOS记录号	WOS:000668973500005
出版者	ELSEVIER
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176997
专题	实验海洋生物学重点实验室
通讯作者	Sun, Chaomin
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Ma, Ning,Cai, Ruining,Sun, Chaomin. Threonine dehydratase enhances bacterial cadmium resistance via driving cysteine desulfuration and biomineralization of cadmium sulfide nanocrystals[J]. JOURNAL OF HAZARDOUS MATERIALS,2021,417:10.
APA	Ma, Ning,Cai, Ruining,&Sun, Chaomin.(2021).Threonine dehydratase enhances bacterial cadmium resistance via driving cysteine desulfuration and biomineralization of cadmium sulfide nanocrystals.JOURNAL OF HAZARDOUS MATERIALS,417,10.
MLA	Ma, Ning,et al."Threonine dehydratase enhances bacterial cadmium resistance via driving cysteine desulfuration and biomineralization of cadmium sulfide nanocrystals".JOURNAL OF HAZARDOUS MATERIALS 417(2021):10.

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