Molecular characterization of harmful algal blooms in the Bohai Sea using metabarcoding analysis

doi:10.1016/j.hal.2021.102066

	Molecular characterization of harmful algal blooms in the Bohai Sea using metabarcoding analysis
	Huang, Hailong 1,2,3; Xu, Qing 1,2,3,5; Gibson, Kate 4; Chen, Yang1,2,3 ; Chen, Nansheng 1,2,3,4
	2021-06-01
发表期刊	HARMFUL ALGAE
ISSN	1568-9883
卷号	106 页码:15
通讯作者	Chen, Nansheng(chenn@qdio.ac.cn)
摘要	Although the occurrences of harmful algal blooms (HABs) have been intensifying, many HABs in coastal waters may have been neglected despite their damaging impact directly on ecology and indirectly on human and animal health. The current detection of HABs depends primarily on the water coloration, chlorophyll intensity, cell density, and mortality due to HAB toxicity. Such methods may not be adequately sensitive to detecting HABs that are relatively transient or small scale. The Bohai Sea is the largest inlet of the Yellow Sea located on the northeast coast of China and famous for shipping and marine aquacultures. HABs frequently occur in the Bohai Sea. In this study, we explored the composition, diversity, and distribution of HAB species using the metabarcoding approach. Through sequencing and the analyzing the 18S rDNA V4 region of 15 samples collected from spatially isolated sites in the Bohai Sea during an expedition in the summer of 2019, we identified 74 potential HAB species including 34 that had not been reported in the Bohai Sea in previous studies. This project provided a detailed analysis of phytoplankton composition, and molecular detection of HAB species in the Bohai Sea. In particular, these analyses revealed extremely high relative abundances of the ichthyotoxic phytoplankton species Vicicitus globosus (Dictyochophyceae) at multiple adjacent sampling sites in the Bohai Bay, which were close to the Yellow River Estuary during the expedition. The results revealed the occurrence of a potential HAB event that would be otherwise undetected using conventional methods, highlighting the sensitivity and power of metabarcoding analysis in detecting HABs and HAB species. This research suggested the value for routine and longterm monitoring of HAB species as an approach for monitoring HABs.
关键词	Harmful algal bloom species Metabarcoding approach 18S rDNA Amplicon sequence variant (ASV) Bohai Sea
DOI	10.1016/j.hal.2021.102066
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000] ; Chinese Academy of Sciences Pioneer Hundred Talents Program ; Taishan Scholar Project Special Fund ; Qingdao Innovation and Creation Plan (Talent Development Program - 5th Annual Pioneer and Innovator Leadership Award)[19-3-2-16-zhc] ; Post-doctoral Program of Qingdao 2020[E0KY061] ; Natural Science Foundation of Shandong Province, China[ZR2020QD095] ; National Natural Science Foundation of China (NSFC) Open Research Cruise - Shiptime Sharing Project of NSFC[NORC2019-01]
WOS研究方向	Marine & Freshwater Biology
WOS类目	Marine & Freshwater Biology
WOS记录号	WOS:000664418400001
出版者	ELSEVIER
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176952
专题	海洋生态与环境科学重点实验室
通讯作者	Chen, Nansheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada 5.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Huang, Hailong,Xu, Qing,Gibson, Kate,et al. Molecular characterization of harmful algal blooms in the Bohai Sea using metabarcoding analysis[J]. HARMFUL ALGAE,2021,106:15.
APA	Huang, Hailong,Xu, Qing,Gibson, Kate,Chen, Yang,&Chen, Nansheng.(2021).Molecular characterization of harmful algal blooms in the Bohai Sea using metabarcoding analysis.HARMFUL ALGAE,106,15.
MLA	Huang, Hailong,et al."Molecular characterization of harmful algal blooms in the Bohai Sea using metabarcoding analysis".HARMFUL ALGAE 106(2021):15.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
1-s2.0-S156898832100（4079KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览