Triterpenoid saponin biosynthesis genes and their expression patterns during the development of sea cucumber Apostichopus japonicus

doi:10.1007/s00343-020-0209-0

	Triterpenoid saponin biosynthesis genes and their expression patterns during the development of sea cucumber Apostichopus japonicus
	Yang, Yujia 1; Li, Xiaoni 2; Sun, Lina 2
	2021-06-14
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
页码	14
通讯作者	Sun, Lina(sunlina@qdio.ac.cn)
摘要	Sea cucumbers are echinoderm species with a leathery skin and an elongated body. Natural compounds, including saponins, have been previously isolated from sea cucumber. The majority of saponins derived from this organism typically belong to the triterpene glycosides, which exhibit various biological effects (e.g., antifungal, cytotoxic, hemolytic, and cytostatic immunomodulatory activities). In the present study, 30 saponin biosynthesis enzymes, namely 2 AACTs, 2 FPSs, 2 HMGSs, 2 OSCs, 1 SS, 2 SEs, and 19 UGTs, were found in the genome of sea cucumber Apostichopus japonicus. Compared with upstream saponin biosynthesis enzymes (i.e., AACTs, FPSs, HMGSs, OSCs, and SSs), the downstream saponin biosynthesis enzymes (SEs and UGTs) shared lower amino acid sequence similarity with the corresponding genes in other echinoderms. It was proposed that the enzyme structures comprised both alpha-helices and beta-sheets. Notably, only a low percentage of beta-sheets were present in FPSs, OSCs, and SS. The saponin biosynthesis enzymes showed dynamic expression patterns during five critical developmental stages of A. japonicus (fertilized oocytes, blastula, gastrula, doliolaria, and penractula). The present study involved elucidation of putative saponin biosynthesis pathways in sea cucumber and provides a valuable platform for further investigation of saponin biosynthesis in echinoderms.
关键词	triterpene saponin biosynthesis sea cucumber enzyme expression development
DOI	10.1007/s00343-020-0209-0
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0900105] ; Agricultural Seed Project of Shandong Province[2017LZGC010] ; Youth Innovation Promotion Association CAS[2019209]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000661406900001
出版者	SCIENCE PRESS
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176880
专题	海洋生态与环境科学重点实验室
通讯作者	Sun, Lina
作者单位	1.Ocean Univ China, Inst Evolut & Marine Biodivers, Lab Evolut & Dev, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Yang, Yujia,Li, Xiaoni,Sun, Lina. Triterpenoid saponin biosynthesis genes and their expression patterns during the development of sea cucumber Apostichopus japonicus[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2021:14.
APA	Yang, Yujia,Li, Xiaoni,&Sun, Lina.(2021).Triterpenoid saponin biosynthesis genes and their expression patterns during the development of sea cucumber Apostichopus japonicus.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,14.
MLA	Yang, Yujia,et al."Triterpenoid saponin biosynthesis genes and their expression patterns during the development of sea cucumber Apostichopus japonicus".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2021):14.

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