Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Actinonin resistance of pathogenic Vibrio anguillarum in aquaculture | |
Yang, Na1,2; Song, Fuhang3; Polyak, Steven W.4; Liu, Jianguo1,2 | |
2021-08-30 | |
发表期刊 | AQUACULTURE |
ISSN | 0044-8486 |
卷号 | 541页码:8 |
通讯作者 | Song, Fuhang(songfuhang@btbu.edu.cn) ; Liu, Jianguo(jgliu@qdio.ac.cn) |
摘要 | Drug-resistant bacteria are an increasingly serious threat to the aquaculture industry. In order to develop new methods to combat these bacteria, it is necessary to first understand their mechanisms of drug resistance. We previously isolated a potential probiotic strain, Streptomyces sp. NHF165, which produced cytotoxic actinonin against the aquaculture pathogen Vibrio anguillarum. In this study, a spontaneous V. anguillarum mutant with an eight-fold increased MIC for actinonin was characterized for elucidation of actinonin resistance mechanisms. Transcriptomic analysis revealed significant up-regulation of genes related to anaerobic respiratory (nap and tor) and down-regulation of genes involved in anaerobic respiration (nqr, sdh and frd) in mutant strains. Meanwhile, no mutations in bacterial respiration genes or translation initiation genes (the proposed actinonin targeted pathway) were detected by whole genome resequencing analysis. A series of experiments confirmed that actinonin-resistance in the mutant strain was associated with respiration. When aerobic respiration was chemically disrupted by CCCP, the growth of wild-type V. anguillarum changed significantly, with a 11.74-fold increase in cell density under actinonin treatment. For anaerobic respiration, an increased nitrate reductase activity was detected in mutant cells by addition of an exogenous electron donor. Noteworthy was the finding that nitrate inhibited the growth of the wild-type strain but promoted the growth of the mutant. Futhermore, the product of nitrate-dependent anaerobic respiration, nitrite, did not arrest the growth of mutant V. anguillarum, which was contrary to previous reports and might exacerbate the formation of vibriosis. Finally, we showed that chemical inhibition of nitrate reductase could restore the susceptibility of mutant V. anguillarum to actinonin. Together these data demonstrate that the combined use of an anaerobic-respiratory inhibitor and an antibiotic might be a promising approach for the treatment of drug-resistant bacteria in aquaculture. |
关键词 | Aquaculture pathogen Vibrio anguillarum Antibiotic resistance mechanisms Transcriptome Respiration Actinonin |
DOI | 10.1016/j.aquaculture.2021.736850 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[31600136] ; Young Scientists Fund of LMBB, Pilot National Laboratory for Marine Science and Technology (Qingdao), China[YQ2018NO04] ; Shandong Provincial Natural Science Foundation, China[ZR2016CB13] ; Research Foundation for Advanced Talents of Beijing Technology and Business University[19008020158] |
WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
WOS类目 | Fisheries ; Marine & Freshwater Biology |
WOS记录号 | WOS:000657609900003 |
出版者 | ELSEVIER |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/176847 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Song, Fuhang; Liu, Jianguo |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Beijing Technol & Business Univ, Sch Light Ind, Beijing, Peoples R China 4.Univ South Australia, UniSA Clin & Hlth Sci, Adelaide, SA, Australia |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Yang, Na,Song, Fuhang,Polyak, Steven W.,et al. Actinonin resistance of pathogenic Vibrio anguillarum in aquaculture[J]. AQUACULTURE,2021,541:8. |
APA | Yang, Na,Song, Fuhang,Polyak, Steven W.,&Liu, Jianguo.(2021).Actinonin resistance of pathogenic Vibrio anguillarum in aquaculture.AQUACULTURE,541,8. |
MLA | Yang, Na,et al."Actinonin resistance of pathogenic Vibrio anguillarum in aquaculture".AQUACULTURE 541(2021):8. |
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