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Actinonin resistance of pathogenic Vibrio anguillarum in aquaculture
Yang, Na1,2; Song, Fuhang3; Polyak, Steven W.4; Liu, Jianguo1,2
2021-08-30
发表期刊AQUACULTURE
ISSN0044-8486
卷号541页码:8
通讯作者Song, Fuhang(songfuhang@btbu.edu.cn) ; Liu, Jianguo(jgliu@qdio.ac.cn)
摘要Drug-resistant bacteria are an increasingly serious threat to the aquaculture industry. In order to develop new methods to combat these bacteria, it is necessary to first understand their mechanisms of drug resistance. We previously isolated a potential probiotic strain, Streptomyces sp. NHF165, which produced cytotoxic actinonin against the aquaculture pathogen Vibrio anguillarum. In this study, a spontaneous V. anguillarum mutant with an eight-fold increased MIC for actinonin was characterized for elucidation of actinonin resistance mechanisms. Transcriptomic analysis revealed significant up-regulation of genes related to anaerobic respiratory (nap and tor) and down-regulation of genes involved in anaerobic respiration (nqr, sdh and frd) in mutant strains. Meanwhile, no mutations in bacterial respiration genes or translation initiation genes (the proposed actinonin targeted pathway) were detected by whole genome resequencing analysis. A series of experiments confirmed that actinonin-resistance in the mutant strain was associated with respiration. When aerobic respiration was chemically disrupted by CCCP, the growth of wild-type V. anguillarum changed significantly, with a 11.74-fold increase in cell density under actinonin treatment. For anaerobic respiration, an increased nitrate reductase activity was detected in mutant cells by addition of an exogenous electron donor. Noteworthy was the finding that nitrate inhibited the growth of the wild-type strain but promoted the growth of the mutant. Futhermore, the product of nitrate-dependent anaerobic respiration, nitrite, did not arrest the growth of mutant V. anguillarum, which was contrary to previous reports and might exacerbate the formation of vibriosis. Finally, we showed that chemical inhibition of nitrate reductase could restore the susceptibility of mutant V. anguillarum to actinonin. Together these data demonstrate that the combined use of an anaerobic-respiratory inhibitor and an antibiotic might be a promising approach for the treatment of drug-resistant bacteria in aquaculture.
关键词Aquaculture pathogen Vibrio anguillarum Antibiotic resistance mechanisms Transcriptome Respiration Actinonin
DOI10.1016/j.aquaculture.2021.736850
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[31600136] ; Young Scientists Fund of LMBB, Pilot National Laboratory for Marine Science and Technology (Qingdao), China[YQ2018NO04] ; Shandong Provincial Natural Science Foundation, China[ZR2016CB13] ; Research Foundation for Advanced Talents of Beijing Technology and Business University[19008020158]
WOS研究方向Fisheries ; Marine & Freshwater Biology
WOS类目Fisheries ; Marine & Freshwater Biology
WOS记录号WOS:000657609900003
出版者ELSEVIER
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/176847
专题实验海洋生物学重点实验室
通讯作者Song, Fuhang; Liu, Jianguo
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Beijing Technol & Business Univ, Sch Light Ind, Beijing, Peoples R China
4.Univ South Australia, UniSA Clin & Hlth Sci, Adelaide, SA, Australia
第一作者单位中国科学院海洋研究所
通讯作者单位中国科学院海洋研究所
推荐引用方式
GB/T 7714
Yang, Na,Song, Fuhang,Polyak, Steven W.,et al. Actinonin resistance of pathogenic Vibrio anguillarum in aquaculture[J]. AQUACULTURE,2021,541:8.
APA Yang, Na,Song, Fuhang,Polyak, Steven W.,&Liu, Jianguo.(2021).Actinonin resistance of pathogenic Vibrio anguillarum in aquaculture.AQUACULTURE,541,8.
MLA Yang, Na,et al."Actinonin resistance of pathogenic Vibrio anguillarum in aquaculture".AQUACULTURE 541(2021):8.
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