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The immunostimulatory effects of hydroxypropyltrimethyl ammonium chloride chitosan-carboxymethyl chitosan nanoparticles
Xu, Chaojie1,2,3,4; Xing, Ronge1,2,3; Liu, Song1,2,3; Qin, Yukun1,2,3; Li, Kecheng1,2,3; Yu, Huahua1,2,3; Li, Pengcheng1,2,3
2021-06-30
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume181Pages:398-409
Corresponding AuthorLi, Pengcheng(pcli@qdio.ac.cn)
AbstractIn this study, we generated chitosan nanoparticles by exploiting the electrostatic interactions between positively charged hydroxypropyltrimethyl ammonium chloride chitosan (HACC) and negatively charged carboxymethyl chitosan (CMC), and examined the effects of altering the molecular weight and carboxymethyl substitution sites of the chitosan molecules. Particle size, potential, and encapsulation efficiency of the various chitosan nano particles were examined; the particle size range was 162.40 & ndash;332.80 nm, the charge range was 19.50 & ndash;40.60 mV, and the encapsulation efficiency range was 48.4 & ndash;70.7%. We then examined the immunostimulatory effects of the nanoparticle variants on dendritic cells (DCs); we found that the site of carboxymethyl substitution significantly affected the immunostimulatory effects of the nanoparticles. Two nanoparticle types, 200 kDa N,O-carboxymethyl chitosan-HACC (NO-CMC-HACC) and N-carboxymethyl chitosan-HACC (N-CMC-HACC), greatly promoted the expression of interleukin-6, tumor necrosis factor, and interleukin-1 beta in DCs. Moreover, NO-CMC-HACC nanoparticles caused an increase in major histocompatibility complex-II (MHC-II), CD11c, CD80, and CD86 secretion in DCs, indicating that these nanoparticles promoted antigen presentation. We then examined chitosan nanoparticle uptake by DCs using laser confocal microscopy; we found that the NO-CMC-HACC nanoparticles were more readily absorbed by DCs compared to the N-CMC-HACC nanoparticles. Therefore, we concluded that 200 kDa NO-CMCHACC nanoparticles exhibited strong potential as immunological adjuvants. (c) 2021 Elsevier B.V. All rights reserved.
KeywordCarboxymethyl chitosan DCS cells Hydroxypropyltrimethyl ammonium chloride chitosan Immunostimulatory activities Nanoparticles
DOI10.1016/j.ijbiomac.2021.03.148
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2018YFC0311300] ; Key Research and Development Program of Shandong Province[2019GHY112015] ; Key Research and Development Program of Shandong Province[2019YYSP028]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000656912700007
PublisherELSEVIER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/176844
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Pengcheng
Affiliation1.Chinese Acad Sci, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Ctr Ocean Megasci, Qingdao, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Xu, Chaojie,Xing, Ronge,Liu, Song,et al. The immunostimulatory effects of hydroxypropyltrimethyl ammonium chloride chitosan-carboxymethyl chitosan nanoparticles[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,181:398-409.
APA Xu, Chaojie.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2021).The immunostimulatory effects of hydroxypropyltrimethyl ammonium chloride chitosan-carboxymethyl chitosan nanoparticles.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,181,398-409.
MLA Xu, Chaojie,et al."The immunostimulatory effects of hydroxypropyltrimethyl ammonium chloride chitosan-carboxymethyl chitosan nanoparticles".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 181(2021):398-409.
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