A deep-sea pathogenic Bacillus subtilis isolate employs different strategies to escape the killing of teleost and murine complements

doi:10.1016/j.dci.2021.104037

IOCAS-IR > 实验海洋生物学重点实验室

	A deep-sea pathogenic Bacillus subtilis isolate employs different strategies to escape the killing of teleost and murine complements
	Gu, Han-Jie 1,2; Li, Mo-Fei1,3 ; Sun, Li 1,3,4
	2021-06-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	119 页码:8
通讯作者	Li, Mo-Fei(murphy210@163.com) ; Sun, Li(lsun@qdio.ac.cn)
摘要	Bacillus subtilis subsp. subtilis G7 was isolated from a deep-sea hydrothermal vent and is pathogenic to pathogenic to fish (Japanese flounder) and mice. G7 is able to survive in host sera and phagocytes. In this study, we investigated the underlying mechanism of G7 serum resistance. We found that (i) the remaining complement activity was very low in G7-incubated flounder serum but high in G7-incubated mouse serum; (ii) cleaved C3 and C5 components were detected on flounder serum-incubated G7 but not on mouse serum-incubated G7; (iii) abundant uncleaved C5 was localized in G7-incubated mouse, but not flounder, serum; (iv) G7-incubated flounder, but not mouse, serum exhibited strong chemotactic activity; (v) pre-treatment with low-dose lysozyme abolished the serum resistance of G7. Hence, G7 activates flounder complement but is protected from complement-mediated destruction by its cell wall structure, while G7 prevents the activation of mouse complement. These results indicate that G7 employs different mechanisms to avoid the complement killing of different hosts.
关键词	Bacillus subtilis Serum resistance Complement activation Immune evasion
DOI	10.1016/j.dci.2021.104037
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050403] ; Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309] ; CAS Key Laboratory of Molecular Virology & Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences[KLMVI-OP202002] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS类目	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS记录号	WOS:000705082500005
出版者	ELSEVIER SCI LTD
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176664
专题	实验海洋生物学重点实验室
通讯作者	Li, Mo-Fei; Sun, Li
作者单位	1.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Ctr Ocean Megasci, Inst Oceanol, Qingdao, Peoples R China 2.CATAS, Hainan Acad Trop Agr Resource, Inst Trop Biosci & Biotechnol, Haikou, Hainan, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Gu, Han-Jie,Li, Mo-Fei,Sun, Li. A deep-sea pathogenic Bacillus subtilis isolate employs different strategies to escape the killing of teleost and murine complements[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,119:8.
APA	Gu, Han-Jie,Li, Mo-Fei,&Sun, Li.(2021).A deep-sea pathogenic Bacillus subtilis isolate employs different strategies to escape the killing of teleost and murine complements.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,119,8.
MLA	Gu, Han-Jie,et al."A deep-sea pathogenic Bacillus subtilis isolate employs different strategies to escape the killing of teleost and murine complements".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 119(2021):8.

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