IOCAS-IR
Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5
Wei, Maosheng1,2,3,4; Geng, Lihua1,2,3; Wang, Qingchi1,2,3,4; Yue, Yang1,2,3; Wang, Jing1,2,3; Wu, Ning1,2,3; Wang, Xiaoqing1,2,3,4; Sun, Chaomin1,2,3; Zhang, Quanbin1,2,3
2021-10-31
发表期刊INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
卷号189页码:649-656
通讯作者Geng, Lihua(lhgeng@qdio.ac.cn) ; Zhang, Quanbin(qbzhang@qdio.ac.cn)
摘要Crude exopolysaccharides from extracellular polymeric substances produced by the marine bacterium Bacillus sp. H5 were fractionated using DEAE-Sepharose FF and Sephadex G-75 chromatography. The high molecular weight fraction (89.0 kD) from the neutral fraction was designated EPS5SH; it contained mannose, glucosamine, glucose, and galactose in a molar ratio of 1.00: 0.02: 0.07: 0.02. Infra-red, gas chromatography-mass spectrometry, electrospray ionisation-tandem mass spectrometry analysis and nuclear magnetic resonance revealed EPS5SH was a mannan with alpha-(1-* 4)-Manp, alpha-(1-* 2)-Manp, alpha-(1-* 4, 6)-Manp and 8-terminal-Manp. Preliminary in vitro experiments revealed that EPS5SH significantly upregulated nitric oxide synthesis and release of pro inflammatory factors in murine macrophage RAW264.7 cells. Western blot experiments verified the immunostimulatory effects of EPS5SH through the modulation of the NF-kappa B and MAPK signalling pathways. In conclusion, EPS5SH was a novel immunostimulatory mannan.
关键词Exopolysaccharide Characteristics Immunostimulatory effects
DOI10.1016/j.ijbiomac.2021.08.159
收录类别SCI
语种英语
资助项目China Ocean Mineral Resources RD Association[DY135-B2-14]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号WOS:000702873200006
出版者ELSEVIER
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/176530
专题中国科学院海洋研究所
通讯作者Geng, Lihua; Zhang, Quanbin
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS, Ctr Ocean Megasci, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Ctr Ocean Megasci, Qingdao, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位中国科学院海洋大科学研究中心
通讯作者单位中国科学院海洋大科学研究中心
推荐引用方式
GB/T 7714
Wei, Maosheng,Geng, Lihua,Wang, Qingchi,et al. Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,189:649-656.
APA Wei, Maosheng.,Geng, Lihua.,Wang, Qingchi.,Yue, Yang.,Wang, Jing.,...&Zhang, Quanbin.(2021).Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,189,649-656.
MLA Wei, Maosheng,et al."Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 189(2021):649-656.
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