IOCAS-IR
Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5
Wei, Maosheng1,2,3,4; Geng, Lihua1,2,3; Wang, Qingchi1,2,3,4; Yue, Yang1,2,3; Wang, Jing1,2,3; Wu, Ning1,2,3; Wang, Xiaoqing1,2,3,4; Sun, Chaomin1,2,3; Zhang, Quanbin1,2,3
2021-10-31
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume189Pages:649-656
Corresponding AuthorGeng, Lihua(lhgeng@qdio.ac.cn) ; Zhang, Quanbin(qbzhang@qdio.ac.cn)
AbstractCrude exopolysaccharides from extracellular polymeric substances produced by the marine bacterium Bacillus sp. H5 were fractionated using DEAE-Sepharose FF and Sephadex G-75 chromatography. The high molecular weight fraction (89.0 kD) from the neutral fraction was designated EPS5SH; it contained mannose, glucosamine, glucose, and galactose in a molar ratio of 1.00: 0.02: 0.07: 0.02. Infra-red, gas chromatography-mass spectrometry, electrospray ionisation-tandem mass spectrometry analysis and nuclear magnetic resonance revealed EPS5SH was a mannan with alpha-(1-* 4)-Manp, alpha-(1-* 2)-Manp, alpha-(1-* 4, 6)-Manp and 8-terminal-Manp. Preliminary in vitro experiments revealed that EPS5SH significantly upregulated nitric oxide synthesis and release of pro inflammatory factors in murine macrophage RAW264.7 cells. Western blot experiments verified the immunostimulatory effects of EPS5SH through the modulation of the NF-kappa B and MAPK signalling pathways. In conclusion, EPS5SH was a novel immunostimulatory mannan.
KeywordExopolysaccharide Characteristics Immunostimulatory effects
DOI10.1016/j.ijbiomac.2021.08.159
Indexed BySCI
Language英语
Funding ProjectChina Ocean Mineral Resources RD Association[DY135-B2-14]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000702873200006
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/176530
Collection中国科学院海洋研究所
Corresponding AuthorGeng, Lihua; Zhang, Quanbin
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS, Ctr Ocean Megasci, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Ctr Ocean Megasci, Qingdao, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wei, Maosheng,Geng, Lihua,Wang, Qingchi,et al. Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,189:649-656.
APA Wei, Maosheng.,Geng, Lihua.,Wang, Qingchi.,Yue, Yang.,Wang, Jing.,...&Zhang, Quanbin.(2021).Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,189,649-656.
MLA Wei, Maosheng,et al."Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 189(2021):649-656.
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