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Genome-wide transcriptome profiling and characterization of mannuronan C5-epimerases in Saccharina japonica
Zhang Pengyan1,2,3,4; Lu Chang1,2,4,5; Shao Zhanru1,2,4; Liu Fuli3,4; Yao Jianting1,2,4; Duan Delin1,2,4,6
2021-12-01
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
Volume60Pages:9
Corresponding AuthorDuan Delin(dlduan@qdio.ac.cn)
AbstractAlginate is a linear polysaccharide comprised of 1,4-linked-beta-D-mannuronic acid (M) and alpha-L-guluronic acid (G). As the major cell wall polysaccharide in brown algae, alginate is widely used in the food, pharmaceutical, and chemical industries. Mannuronan C5-epimerases (MC5Es) catalyze the specific conversion of M to its C-5 epimer G, playing a key role in the modification of alginate. In this study, 143 MC5E gene sequences from Saccharina japonica (SjMC5Es) were identified based on the genome and transcriptome data. Transcriptomics analysis was conducted, and the results showed that the SjMC5Es varied in their spatial and temporal expression patterns. Most SjMC5Es exhibited higher expression in the basal blades of the algae at earlier developmental stages than at later adult stages, and the transcription levels of the SjMC5Es showed a clear trend of gradual increase or decrease from the basal to the distal blades. In addition, the beta-D-mannuronic acid/alpha-L-guluronic acid (M/G) ratio of alginate from all samples was measured, and the M/G ratios were associated with the transcription levels of the SjMC5E genes. Finally, the highly expressed SjMC5E2 was characterized, soluble SjMC5E2 was heterologously expressed, and its mannuronan C5-epimerase activity was confirmed by H-1-nuclear magnetic resonance analysis. Our results provide a global view of the SjMC5Es in S. japonica and lay a foundation for further applications of MC5Es in improving the physiochemical properties of alginate.
KeywordAlginate Mannuronan C5-epimerase M/G ratio Saccharina japonica Transcription profile
DOI10.1016/j.algal.2021.102491
Indexed BySCI
Language英语
Funding ProjectJoint Research Project between China and Japan[2017YEE0130900] ; Joint Research Project between China and Korea[2021YFE010146] ; National Nature Science Foundation of China[42006101] ; Open Foundation of State Key Lab of Seaweed Bioactive Substance[SKL-BASS1702]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000696910900002
PublisherELSEVIER
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/176455
Collection实验海洋生物学重点实验室
Corresponding AuthorDuan Delin
Affiliation1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Div Mariculture Ecol & Technol, Qingdao 266071, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.State Key Lab Seaweed Bioact Subst, Qingdao 266000, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang Pengyan,Lu Chang,Shao Zhanru,et al. Genome-wide transcriptome profiling and characterization of mannuronan C5-epimerases in Saccharina japonica[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2021,60:9.
APA Zhang Pengyan,Lu Chang,Shao Zhanru,Liu Fuli,Yao Jianting,&Duan Delin.(2021).Genome-wide transcriptome profiling and characterization of mannuronan C5-epimerases in Saccharina japonica.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,60,9.
MLA Zhang Pengyan,et al."Genome-wide transcriptome profiling and characterization of mannuronan C5-epimerases in Saccharina japonica".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 60(2021):9.
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