Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Exogenous sodium fumarate enhances astaxanthin accumulation in Haematococcus pluvialis by enhancing the respiratory metabolic pathway | |
| Yu, Wenjie1,2,3; Zhang, Litao1,2; Zhao, Jing1,2,4; Liu, Jianguo1,2 | |
| 2021-12-01 | |
| Source Publication | BIORESOURCE TECHNOLOGY
 |
| ISSN | 0960-8524 |
| Volume | 341Pages:9 |
| Corresponding Author | Liu, Jianguo(jgliu@qdio.ac.cn) |
| Abstract | Improvement of astaxanthin yield is a continuing objective in Haematococcus pluvialis cultivation. In this study, a new strategy combining exogenous sodium fumarate (SF) with nitrogen deficiency was demonstrated to promote astaxanthin accumulation in H. pluvialis. SF significantly accelerated astaxanthin synthesis over cell growth. Notably, under nitrogen deficiency, 10 mM SF increased the cellular astaxanthin content by 1.75-fold and 1.47 fold on day 7 and 12, respectively. Further studies indicated that SF increased the level of respiratory metabolites in the Embden-Meyerhof-Parnas pathway, tricarboxylic acid cycle and pentose phosphate pathway. An enhanced substrate pool due to the acceleration of respiratory metabolic pathways directly induced astaxanthin synthesis. Meanwhile, the accumulation of C18:1, C18:2 and C18:3 fatty acids enhanced astaxanthin synthesis indirectly by promoting the process of esterification. This study not only helps to elucidate the role of respiratory metabolic pathways in astaxanthin synthesis but also provides a new effective technique to improve astaxanthin production. |
| Keyword | Astaxanthin Haematococcus pluvialis Sodium fumarate Respiratory metabolic pathway Nitrogen deficiency |
| DOI | 10.1016/j.biortech.2021.125788 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key Research and Development Program of China[2018YFE0107200] ; National Natural Science Foundation of China[31872588] ; National Natural Science Foundation of China[U1706209] ; Shandong Provincial Natural Science Foundation, China[ZR2019BC057] |
| WOS Research Area | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS Subject | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS ID | WOS:000694798100003 |
| Publisher | ELSEVIER SCI LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/176419 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Liu, Jianguo |
| Affiliation | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China |
| First Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Corresponding Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Yu, Wenjie,Zhang, Litao,Zhao, Jing,et al. Exogenous sodium fumarate enhances astaxanthin accumulation in Haematococcus pluvialis by enhancing the respiratory metabolic pathway[J]. BIORESOURCE TECHNOLOGY,2021,341:9. |
| APA | Yu, Wenjie,Zhang, Litao,Zhao, Jing,&Liu, Jianguo.(2021).Exogenous sodium fumarate enhances astaxanthin accumulation in Haematococcus pluvialis by enhancing the respiratory metabolic pathway.BIORESOURCE TECHNOLOGY,341,9. |
| MLA | Yu, Wenjie,et al."Exogenous sodium fumarate enhances astaxanthin accumulation in Haematococcus pluvialis by enhancing the respiratory metabolic pathway".BIORESOURCE TECHNOLOGY 341(2021):9. |
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| File Name/Size | DocType | Version | Access | License | ||
| 1-s2.0-S096085242101（3574KB） | 期刊论文 | 出版稿 | 延迟开放 | CC BY-NC-SA | View 2023-7-1后可获取 | |
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