High-Quality Genome Assembly and Comprehensive Transcriptome of the Painted Lady Butterfly Vanessa cardui

doi:10.1093/gbe/evab145

	High-Quality Genome Assembly and Comprehensive Transcriptome of the Painted Lady Butterfly Vanessa cardui
	Zhang, Linlin 1,2,3,4; Steward, Rachel A.5; Wheat, Christopher W.5; Reed, Robert D.6
	2021-07-01
发表期刊	GENOME BIOLOGY AND EVOLUTION
ISSN	1759-6653
卷号	13 期号:7 页码:7
通讯作者	Zhang, Linlin(linlinzhang@qdio.ac.cn) ; Reed, Robert D.(robertreed@cornell.edu)
摘要	The painted lady butterfly, Vanessa cardui, has the longest migration routes, the widest hostplant diversity, and one of the most complex wing patterns of any insect. Due to minimal culturing requirements, easily characterized wing pattern elements, and technical feasibility of CRISPR/Cas9 genome editing, V. cardui is emerging as a functional genomics model for diverse research programs. Here, we report a high-quality, annotated genome assembly of the V. cardui genome, generated using 84 x coverage of PacBiolong-readdata, which we assembled into 205 contigs with a total length of 425.4 Mb(N50 = 10.3Mb). The genome was very complete (single-copy complete Benchmarking Universal Single-Copy Orthologs [BUSCO] 97%), with contigs assembled into presumptive chromosomes using synteny analyses. Our annotation used embryonic, larval, and pupal transcriptomes, and 20 transcriptomes across five different wing developmental stages. Gene annotations showed a high level of accuracy and completeness, with 14,437 predicted protein-coding genes. This annotated genome assembly constitutes an important resource for diverse functional genomic studies ranging from the developmental genetic basis of butterfly color pattern, to coevolution with diverse hostplants.
关键词	PacBio sequencing de novo genome assembly RNA-seq butterfly wing color patterning
DOI	10.1093/gbe/evab145
收录类别	SCI
语种	英语
资助项目	United States National Science Foundation[IOS-1656514] ; United States National Science Foundation[IOS-1753559] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; National Natural Science Foundation of China[41976088] ; Pilot National Laboratory for Marine Science and Technology[YJ2019NO01] ; Key Development Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Science[COMS2019R01] ; Carl Tryggers Stiftelse anslag[CTS 18:415] ; Swedish Research Council[2017-04386]
WOS研究方向	Evolutionary Biology ; Genetics & Heredity
WOS类目	Evolutionary Biology ; Genetics & Heredity
WOS记录号	WOS:000690270100027
出版者	OXFORD UNIV PRESS
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176306
专题	深海极端环境与生命过程研究中心
通讯作者	Zhang, Linlin; Reed, Robert D.
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 5.Stockholm Univ, Dept Zool, Stockholm, Sweden 6.Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY 14853 USA
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Zhang, Linlin,Steward, Rachel A.,Wheat, Christopher W.,et al. High-Quality Genome Assembly and Comprehensive Transcriptome of the Painted Lady Butterfly Vanessa cardui[J]. GENOME BIOLOGY AND EVOLUTION,2021,13(7):7.
APA	Zhang, Linlin,Steward, Rachel A.,Wheat, Christopher W.,&Reed, Robert D..(2021).High-Quality Genome Assembly and Comprehensive Transcriptome of the Painted Lady Butterfly Vanessa cardui.GENOME BIOLOGY AND EVOLUTION,13(7),7.
MLA	Zhang, Linlin,et al."High-Quality Genome Assembly and Comprehensive Transcriptome of the Painted Lady Butterfly Vanessa cardui".GENOME BIOLOGY AND EVOLUTION 13.7(2021):7.

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