Optimization of extractions of eumelanin from cuttlefish ink and the hypoglycemic effects: In vitro enzyme inhibitory activity and glucose consumption in HepG2 cells

doi:10.1111/jfpp.15868

IOCAS-IR > 实验海洋生物学重点实验室

	Optimization of extractions of eumelanin from cuttlefish ink and the hypoglycemic effects: In vitro enzyme inhibitory activity and glucose consumption in HepG2 cells
	Song, Wen 1,2,3,4; Xing, Rong-e1,2,3 ; Yang, Haoyue1,2,3 ; Liu, Song 1,2,3; Li, Pengcheng1,2,3
	2021-08-25
发表期刊	JOURNAL OF FOOD PROCESSING AND PRESERVATION
ISSN	0145-8892
页码	17
通讯作者	Xing, Rong-e(xingronge@qdio.ac.cn) ; Yang, Haoyue(yanghaoye@qdio.ac.cn)
摘要	Pigment compounds with hypoglycemic effects are a widely researched topic, but there is almost no research evaluation on eumelanin in ink. Melanin in cuttlefish juice is used as an antibacterial, hemostatic, and other active natural medicine and food additive. This study is the first to compare the extraction method of alkali-extracted eumelanin and enzyme-extracted eumelanin and to analyze their hypoglycemic activities. The structures of the extracted samples were analyzed by Fourier Transform infrared spectroscopy, Ultraviolet-visible spectroscopy (UV-vis), X-ray, and scanning electron microscope (SEM). The purity of the extracted samples was determined by high-performance liquid chromatography (HPLC). The results showed that the enzyme extraction yield and purity of eumelanin were 88.3% and 91.62%, and the alkali extraction yield and purity of eumelanin were 16.82% and 58.57%, respectively. Enzyme extraction of eumelanin can significantly inhibit alpha-glycosidase enzyme and alpha-amylase activity, promote the utilization of glucose in cells, and increase hepatic glycogen synthesis. Eumelanin (0.8 mg/ml) was selected as the effective concentration. This study provides a technical reference for the extraction, determination, and hypoglycemic activity of eumelanin. Novelty impact statement This study is the first to compare the extraction method of alkali-assisted extraction of eumelanin and enzymatic-assisted extraction of eumelanin and to analyze their hypoglycemic activities. The extraction yield and purity of enzyme-assisted extraction of eumelanin extraction is much higher than that of alkali-assisted extraction of eumelanin. Eumelanin from squid ink has the effect of accelerating the utilization of glucose and the effect of enzyme-assisted extraction of eumelanin is slightly better than alkali-assisted extraction of eumelanin.
DOI	10.1111/jfpp.15868
收录类别	SCI
语种	英语
资助项目	Chinese Academy of Sciences[COMS2020J04]
WOS研究方向	Food Science & Technology
WOS类目	Food Science & Technology
WOS记录号	WOS:000687992500001
出版者	WILEY
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176282
专题	实验海洋生物学重点实验室
通讯作者	Xing, Rong-e; Yang, Haoyue
作者单位	1.AS, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Sch Earth & Planetary, Beijing, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Song, Wen,Xing, Rong-e,Yang, Haoyue,et al. Optimization of extractions of eumelanin from cuttlefish ink and the hypoglycemic effects: In vitro enzyme inhibitory activity and glucose consumption in HepG2 cells[J]. JOURNAL OF FOOD PROCESSING AND PRESERVATION,2021:17.
APA	Song, Wen,Xing, Rong-e,Yang, Haoyue,Liu, Song,&Li, Pengcheng.(2021).Optimization of extractions of eumelanin from cuttlefish ink and the hypoglycemic effects: In vitro enzyme inhibitory activity and glucose consumption in HepG2 cells.JOURNAL OF FOOD PROCESSING AND PRESERVATION,17.
MLA	Song, Wen,et al."Optimization of extractions of eumelanin from cuttlefish ink and the hypoglycemic effects: In vitro enzyme inhibitory activity and glucose consumption in HepG2 cells".JOURNAL OF FOOD PROCESSING AND PRESERVATION (2021):17.

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