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| Differential proteomic analysis by iTRAQ reveals the growth mechanism in Pyropia yezoensis mutant | |
Ma, Yingchao1,2,3; He, Bangxiang2,4,5; Wang, Xulei2,4 ; He, Linwen2,4; Niu, Jianfeng2,4; Huan, Li2,4; Lu, Xiaoping2,4; Xie, Xiujun2,4; Wang, Guangce2,4
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| 2021-10-01 | |
| 发表期刊 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
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| ISSN | 2211-9264 |
| 卷号 | 58页码:8 |
| 通讯作者 | Wang, Guangce(gcwang@qdio.ac.cn) |
| 摘要 | The previous study revealed that the mutant strain E thalli of Pyropia yezoensis had growth advantage compared with the initial strain NA, and transcriptome analysis revealed the relevant bioprocesses for improved growth. To investigate the growth mechanism at the protein level, this study used isobaric tags for relative and absolute quantification and liquid chromatography-mass spectrometry to analyze proteome of both strains. In total, 277 proteins were identified, among which 41 differentially expressed proteins were screened, including 22 up regulated proteins and 19 down-regulated proteins. Integrated analysis of proteome and previous transcriptome showed that the proteins related to photosynthesis, protein biosynthesis, glycolysis, and pentose phosphate pathway were enhanced in mutant strain E, which were supposed to facilitate growth of mutant strain E, and the proteins relevant to stress resistance were up-regulated, which might promote the growth of mutant strain E under abiotic stresses, while proteins related to methylation were decreased, which might enhance the expression of growth relevant genes in mutant strain E. This study revealed the growth mechanism in mutant strain E by integrated omics analysis, and also provided theoretical foundation for molecular breeding of Py. yezoensis. |
| 关键词 | Pyropia yezoensis Growth mechanism Differentially expressed proteins iTRAQ |
| DOI | 10.1016/j.algal.2021.102420 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Key Research and Devel-opment Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41876163] ; National Natural Science Foundation of China[41776150] ; National Natural Science Foundation of China[41876160] ; National Natural Science Foundation of China[41906105] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2019Q02] ; Major Scientific and Technological Innovation Projects in Shandong Province[2019JZZY010815] ; North Jiangsu Science and Technol-ogy Special Project[LYGSZ201916] ; Science and Technology Project of Dongtou District[N2016Y18A] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000686735100006 |
| 出版者 | ELSEVIER |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/176279 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Guangce |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Marine & Environm Sci, Asian Reg Artemia Reference Ctr, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Minist Educ, Key Lab Marine Resource Chem & Food Technol TUST, Tianjin 300457, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, China Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 5.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Ma, Yingchao,He, Bangxiang,Wang, Xulei,et al. Differential proteomic analysis by iTRAQ reveals the growth mechanism in Pyropia yezoensis mutant[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2021,58:8. |
| APA | Ma, Yingchao.,He, Bangxiang.,Wang, Xulei.,He, Linwen.,Niu, Jianfeng.,...&Wang, Guangce.(2021).Differential proteomic analysis by iTRAQ reveals the growth mechanism in Pyropia yezoensis mutant.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,58,8. |
| MLA | Ma, Yingchao,et al."Differential proteomic analysis by iTRAQ reveals the growth mechanism in Pyropia yezoensis mutant".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 58(2021):8. |
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| 1-s2.0-S221192642100（4733KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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