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An inactivated bivalent vaccine effectively protects turbot (Scophthalmus maximus) against Vibrio anguillarum and Vibrio harveyi infection
Zhang, Jian1,2,3; Hu, Yonghua3,4,5; Sun, Qinglei1,3; Li, Xuepeng2; Sun, Li1,3
2021-11-15
Source PublicationAQUACULTURE
ISSN0044-8486
Volume544Pages:7
Abstract

Vibrio anguillarum and Vibrio harveyi are two important pathogens that affect a wide range of fish species. In this study, we developed a formalin-inactivated whole-cell bivalent vaccine (named IVVah1) against V. anguillarum and V. harveyi and examined its effect in a turbot model. The results showed that when administered via a single intraperitoneal (i.p.) injection, the vaccine induced high rates of relative percentage survival (RPS) against V. anguillarum and V. harveyi from the third week post-vaccination (wpv). The RPS reached 94.74% and 91.89% against V. anguillarum and V. harveyi, respectively, at 4 wpv, and remained high (>80%) till at least 8 wpv. Specific antibodies were produced in the vaccinated fish at 1 wpv and peaked at 2 to 3 wpv. Secondary antigen exposure induced strong proliferation of peripheral blood leukocytes (PBLs) and increased the number of monocytes in PBLs. Compared to the control fish, the vaccinated fish exhibited normal growth rates and were able to thoroughly clear the challenging bacterial pathogens from tissues. These findings indicate that IVVah1 is a highly protective bivalent vaccine with a good potential to be used in aquaculture against V. anguillarum and V. harveyi infection.

KeywordBivalent vaccine Vibriosis Relative percentage survival (RPS) Specific immune response Monocytes
DOI10.1016/j.aquaculture.2021.737158
Indexed BySCI
Language英语
Funding ProjectMarine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ05023] ; National Marine Economy Innovation and Development Regional Demonstration Project (Qingdao) ; Taishan Scholar Program of Shandong Province
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000685093200003
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/176026
Collection实验海洋生物学重点实验室
Corresponding AuthorZhang, Jian; Sun, Li
Affiliation1.Chinese Acad Sci, CAS Ctr Ocean Mega Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Yantai Univ, Sch Ocean, Yantai, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Hainan Acad Trop Agr Resource, CATAS, Inst Trop Biosci & Biotechnol, Haikou, Hainan, Peoples R China
5.Hainan Prov Key Lab Funct Components Res & Utiliz, Haikou 571101, Hainan, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Jian,Hu, Yonghua,Sun, Qinglei,et al. An inactivated bivalent vaccine effectively protects turbot (Scophthalmus maximus) against Vibrio anguillarum and Vibrio harveyi infection[J]. AQUACULTURE,2021,544:7.
APA Zhang, Jian,Hu, Yonghua,Sun, Qinglei,Li, Xuepeng,&Sun, Li.(2021).An inactivated bivalent vaccine effectively protects turbot (Scophthalmus maximus) against Vibrio anguillarum and Vibrio harveyi infection.AQUACULTURE,544,7.
MLA Zhang, Jian,et al."An inactivated bivalent vaccine effectively protects turbot (Scophthalmus maximus) against Vibrio anguillarum and Vibrio harveyi infection".AQUACULTURE 544(2021):7.
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